Fig. 3

Mitochondrial protein content in young and age, male and female mice. A Representative western blot from male (top panel) and female (bottom panel) mice for OXPHOS protein. B–F Quantification of each independent mitochondrial protein. B Complex-V protein (ATP5A) protein, C Complex-III (UQCRC2) protein, D Complex-IV (MTCO1) protein, E Complex-II (SDH8), and F Complex I (NDUFB8) protein. G Quantification of total OXPHOS. All values were corrected to Ponceau stain (P.S), and values are reported as means ± SEM, in A.U. The main effects of 2-way ANOVA are represented on the graph at p < 0.05. δ p < 0.05, post hoc significance. *p < 0.05, t-test between indicated groups. N = 10/male group, 8/female group