Fig. 1
From: High-throughput muscle fiber typing from RNA sequencing data
A single-nuclei RNAseq of the human skeletal muscle. Slow- (type I) and fast-twitch (type II) fibers form species-specific distinct clusters of nuclei. a Five major clusters of nuclei were identified using graph-based clustering built on Louvain modularity optimization. For visualization of the nuclei populations, t-distributed stochastic neighbor embedding (tSNE) non-linear dimension reduction was applied. b Examples of nuclei expression patterns for genes separating different clusters, i.e., ATP2A1, MYBPC2, and MYH2 are enriched in cluster A (type II fiber), XPO4, ATP2A2, TPM3, and MYH7B are enriched in cluster B (type I fiber), LRRTM4 is enriched in cluster D (endothelial), and MECOM is enriched in cluster E. c Complete list of the 48 marker genes separating the five clusters