Fig. 1

Elevated nuclear p53 and progerin are closely related to cell premature senescence in case of denervated muscle atrophy. A Elevated expression of the P53 mRNA in the WT group of mice after denervation was determined by qPCR. The level of elevation of P53 in mice in the Den group was much higher than that of mice in the control group. ***P<0.001 versus control, ****P<0.0001 versus control (n=6/group). B P53 protein levels determined by western blotting showed a trend similar to that of the mRNA expression. *P<0.05 versus control (n=6/group). C Compared with the muscles of mice in the control group, the positive expression of p53 increased in the denervated muscles of the Den-2w group of mice. D The elevated expression of progerin mRNA in the WT group after denervation was determined by qPCR. Elevation in progerin levels of mice in the Den-2w group was much higher than that of the control group. **P<0.01 versus control, ****P<0.0001 versus control (n=6/group). E Progerin protein levels determined by western blotting showed a trend similar to that of mRNA expression. *P<0.05 versus control, **P<0.01 versus control (n=6/group). F Compared with mice in the control group, the positive expression of progerin increased in the denervated muscle of mice in the Den-2w group. G–H Compared with muscles of mice in the control group, the positive expression of SASP-related factors such as IL-1 and IL-6 increased in the denervated muscles of the Den-2w group of mice. I The co-localization of p53 (red) with progerin (green) in the denervated muscle, shown by immunofluorescence. J Endogenous co-IP showed the positive interaction of p53 and progerin in denervated muscle. K ß-gal activity combined with p21 in sequential sections showed skeletal muscle cellular senescence. Scale bar, 50 μm