Fig. 2
From: Mouse models of SMA show divergent patterns of neuronal vulnerability and resilience
Significant motor neurons loss is observed in thoracic spinal cord segments in the Smn2B/− mouse model. A Representative images showing motor neurons (MNs) labelled with an antibody against choline acetyltransferase (ChAT; magenta), as well as Nissl (green) and DAPI (blue) staining in Smn2B/− and Smn2B/+ control mice in thoracic spinal cord segments (T5 and T11) and lumbar spinal cord segment L5 at P16. Scale bar: 50μm. B–D Bar charts showing the total number of MNs quantified in the ventral horn in the spinal cord segments T5, T11 and L5 in Smn2B/− mice compared to Smn2B/+ controls. There was a significant decrease in the total number of MNs in Smn2B/− mice compared to control in both thoracic spinal cord segments. However, there was no significant difference in the total number of MNs in the lumbar segment. Unpaired Student’s t test (ns= no significance, *p≤0.05). n=3,4 for controls and Smn2B/− mice respectively in T5; n=4 for both genotypes in all other spinal cord segments. Error bars represent mean ± SEM