Fig. 7

Simvastatin failed to downregulate HMGCR and LDLR signaling in skeletal muscles in Chow or HFD-fed Dysf-null mice. A Representative images and immunoblot quantification of HMGCR and LDLR in gastrocnemius muscles of Chow-fed, WT and Dysf mice treated with either vehicle or simvastatin. Two-way ANOVA with Sidak’s post hoc tests were used for direct mean comparisons; *P < 0.05, **P < 0.01, and ****P < 0.0001. B Representative images and immunoblot quantification of HMGCR and LDLR in gastrocnemius muscles of Chow-fed, WT and Dysf mice treated with either vehicle or simvastatin. Two-way ANOVA with Sidak’s post hoc tests were used for direct mean comparisons; *P < 0.05, **P < 0.01, and ****P < 0.0001. HMGCR and LDLR were standardized to the loading control GAPDH. Y-axes represent arbitrary units (AU). Full blots were imaged separately and thus have differing exposures. A common sample was loading onto each gel to normalize for detection efficiencies across membranes. Mean ± SEM. N = 5 for each cohorts