Fig. 8

Simvastatin failed to normalize intramuscular levels of Filipin and HMGCR in the quadriceps of Chow or HFD-fed Dysf-null mice. A Representative images of Filipin and HMGCR in both Chow and HFD-fed, WT and Dysf mice treated with either vehicle or simvastatin. B Quantification of the average fluorescent intensity of Filipin and HMGCR. Relative intensity values were generated by normalizing to WT (Chow) levels to account for detection efficiencies. Scale 50 μm in both main images and insets. Insets: no Filipin control (DRAQ7 only) and no HMGCR control DAPI only). Two-way ANOVA with Sidak’s post hoc tests were used for direct comparisons between WT and Dysf mice; *P < 0.05; **P < 0.01; ***P < 0.001. Mean ± SEM. N = 4–7 per cohort