Fig. 2

New functionalities of the plugin. A Immunostaining of skeletal muscle with WGA showing the extracellular matrix (ECM) in green (SB = 600 µm) and respective quantification with MuscleJ2 in the "GlobalResults" file. B Immunostaining of skeletal muscle with laminin (gray) and CD31 showing the endothelial cells in red (SB = 600 µm) and quantification of vessels and capillaries with MuscleJ2. Tables present the results obtained after selecting the option Vascularization (section Data Analysis by Section) and Capillaries (section Data Analysis by Fibers). The gray table presents the results obtained in the "GlobalResults" file, and the green table presents some of the results obtained in the "CapillaryDetails" file (SB = 600 µm). C Immunostaining of skeletal muscle with laminin (gray), DAPI (blue), and F4/80 showing the macrophages in red and quantification of specific cells with MuscleJ2 (SB = 600 µm). The gray table presents the results obtained in the "GlobalResults" file, and the green table presents some of the results obtained in the "SpecificCells" file. Nucleus GC X and Y correspond to the coordinates of identified specific cells colabeled with DAPI. All areas are indicated in µm²