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Fig. 3 | Skeletal Muscle

Fig. 3

From: MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides

Fig. 3

Measurement of Fiber Intensity by ROI. A Representation of the different ROIs in MuscleJ2. ROIF, ROI Fiber; ROICNF, ROI Centronucleated Fiber; ROISC, ROI Satellite Cell; ROICap, ROI Capillary; ROIMB, ROI Membrane. B Original image of skeletal muscle stained with dystrophin and corresponding cartographies representing the different ROIs obtained after MuscleJ2 analysis with the Fiber Intensity option. C For each fiber, the intensity of the staining and the percentage of positive pixels in each ROI are given. D Quantification of dystrophin staining in the different ROIs. The gray table presents the results obtained in the "GlobalResults" file, and the green table presents some of the results obtained in the "FiberDetails" file

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