Fig. 1

Generation of human iPSC-myotubes. A Schematic of differentiation of human iPSC myotubes using protocol described in Chal et al. [19] and Hicks et al. [18]. Skeletal muscle progenitor cells (SMPCs) are differentiated from iPSCs and enriched by flow sorting. The SMPCs are then expanded in SKGM2 media and differentiated to form myotubes. B Gene expression analysis by qRT-PCR of undifferentiated SMPCs (D0) and day 4 differentiated myotubes (D4). C Phase-contrast picture and immunocytochemistry staining of day 4 differentiated iPSC myotubes. MYHC, pan myosin heavy chain; MYH3, myosin heavy chain 3. Scale bar — 10 μm (phase) and 20 μm (fluorescence)