Fig. 3

Motor neurons promote alignment of skeletal myocytes. The orientation of skeletal myocytes across all the groups — MYO + MN + EC, MYO + MN, MYO + EC, and MYO, was measured using directionality plug-in in ImageJ. The angle of orientation from three replicates per group was plotted as histograms. Cultures with motor neurons (i.e., MYO + MN and MYO + MN + EC) promoted the formation of anisotropic clusters of myofibers. The yellow broken lines indicate the reference orientation of the central 0-degree angle (horizontal line and the vertical are 90°). Scale bar — 500 μm