Table 2 Details of primers for gene expression analysis
Group | Gene | Primer (forward) | Primer (reverse) |
|---|---|---|---|
Myosin heavy chain isoforms | MYH1 | AATGTCCAAGGCCAACAGTGA | AGCATCCTGCAGACGCTGA |
MYH2 | GGAGGACAAAGTCAACACCCTG | GCCCTTTCTAGGTCCATGCGAA | |
MYH3 | CTGGAGGATGAATGCTCAGAGC | CCCAGAGAGTTCCTCAGTAAGG | |
MYH4 | GACAGCCAAGAAGAGGAAACTGG | ACCTGCCATCTCTTCTGTGAGG | |
MYH6 | GGAAGACAAGGTCAACAGCCTG | TCCAGTTTCCGCTTTGCTCGCT | |
MYH7 | GGAGTTCACACGCCTCAAAGAG | TCCTCAGCATCTGCCAGGTTGT | |
MYH8 | GGAGCAAGCTGAGCCAGATG | CACAGTCTGGCCTTTGGTGA | |
Developmental | Pax7 | GGAGGATGAAGCGGACAAGAAG | AGGTCAGGTTCCGACTCCACAT |
MYOD1 | CTCCAACTGCTCCGACGGCAT | ACAGGCAGTCTAGGCTCGACAC | |
MYOG | AGTGCCATCCAGTACATCGAGC | AGGCGCTGTGAGAGCTGCATTC | |
DMD | GCTCAACCATCGATTTGCAGCC | TTCAGCCTCCAGTGGTTCAAGC | |
Structural | TNNT1 | AACGCGAACGTCAGGCTAAGCT | CTTGACCAGGTAGCCGCCAAAA |
TTN | CTGCTGACTACACCTTTGTGGC | GCTCGCTTCTTCTCCAGTACCT | |
Glucose transporter | SLC2A1 | TTGCAGGCTTCTCCAACTGGAC | CAGAACCAGGAGCACAGTGAAG |
SLC2A4 | CCATCCTGATGACTGTGGCTCT | GCCACGATGAACCAAGGAATGG | |
Neuromuscular | MUSK | TTTGCTGTCCGTGCCAGAATGC | GGCTTTGAGGACGTCATTGGTG |
LRP4 | GTGTGGCAGAACCTTGACAGTC | ACCGCTCTAACTTGGCATTCTCC | |
RAPSYN | GGACAAAGGTGCTGGAGAAGAG | TGTCGATCTGGACCACAGCGAA | |
DOK7 | GCCATCATGCTGGGCTTTGACA | AACTTGGTGCCTGGAGCCACTG |