Fig. 1

The number of FAPs and SCs in the TA muscle increases after hindlimb immobilization. A Micrographs of TA muscle tissue stained with H&E. Muscles were collected from days 0 and 14 after hindlimb immobilization of young (upper) and aged (lower) mouse. Bar, 50 μm. B Frequency distribution of CSA of muscle fibers of TA muscles collected from days 0 and 14 after hindlimb immobilization of young (upper) and aged (lower) mice. Accumulated data from three mice are shown in each group. C Expression levels of Fbxo32 (left) and Trim63 (right) transcripts in the TA muscles from young and aged mice on days 0, 3, and 14 of immobilization (n = 3–4). D Hindlimb muscle weight on days 3 and 14 after hindlimb immobilization in young, adult, and aged mice. E,F Number of FAPs (E) and SCs (F) on days 3 and 14 after hindlimb immobilization (n = 3–10 mice per group). *P < 0.05; **P < 0.01