Fig. 2

Sex-dependent response in mitochondrial content and function to denervation. Cytochrome c oxidase (COX) activity assessed as a measure of mitochondrial content basally (A). COX activity following denervation in male animals (B) and female animals (C). Oxygen consumption measured in permeabilized muscle fibers in the presence of pyruvate + malate, ADP and succinate in male and female mice basally (D). Oxygen consumption in the respective states in male mice following denervation (E) and female mice (F). ROS emission rates corrected for respiration supported by pyruvate + malate, ADP, and succinate basally in male and female mice (G). ROS emission rates under the respective states in male mice following denervation. (H) and female mice (I). Two-way ANOVAs were conducted with repeated measures between CON and DEN conditions, followed by Tukey post-hoc analyses. P + M, pyruvate malate; G, represents a main effect of genotype; S, denotes a main effect of sex; D, indicates a main effect of denervation; SxG, represents an interaction between genotype and sex; *, p < 0.05; **, p < 0.01; ****, p < 0.0001; n = 7