Fig. 3

Females exhibit higher autophagy and mitophagy protein levels and these proteins increase dramatically with denervation in a TFE3-dependent manner. Representative blots for autophagy- and mitophagy-related markers following 7 days (7D) of denervation (A). ATG7 protein content (B) was quantified basally between males and females, then following denervation in male mice and female mice. Similar quantifications were performed for Beclin1 (C), Parkin (D), TFEB (E), and TFE3 (F) protein content. Two-way ANOVAs were conducted with repeated measures between CON and DEN conditions, followed by Tukey post-hoc analyses. G, represents a main effect of genotype; S, denotes a main effect of sex; D, indicates a main effect of denervation; GxS, represents an interaction between genotype and sex; GxS, indicates an interaction effect between genotype and sex; GxS, interaction effect between genotype and sex; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; control n-14-16, denervated n = 7–8