Fig. 4

Mitophagic response to chronic denervation is dependent on sex and influenced byTFE3. Representative blots of mitophagy flux following 7 days of denervation (A). Quantification of LC3-II mitophagy flux, taken as the difference between colchicine- and vehicle-treated conditions (A). LC3-II mitophagic flux was assessed basally in male and female mice (B). LC3-II flux was also measured following 7 days of denervation in male mice (C) and female mice (D). Two-way ANOVAs were conducted with repeated measures between CON and DEN conditions, followed by Tukey post-hoc analyses. G, represents a main effect of genotype; S, denotes a main effect of sex; DxG, interaction effect between denervation and genotype; *, p < 0.05; **, p < 0.01; ***, p < 0.001; control n = 14–16, denervated n = 7–8