Fig. 5

Denervation increases lysosomal markers independent of sex or genotype. Representative blots of lysosomal markers following 7 days of denervation (A). Mature Cathepsin D protein content (B) was quantified basally between males and females, then following denervation in male mice and female mice. Similar quantifications were performed for mature Cathepsin B (C), Cathepsin B ratio (D), v-ATPase (E), and MCOLN1 (F). Two-way ANOVAs were conducted with repeated measures between CON and DEN conditions, followed by Tukey post-hoc analyses. S, denotes a main effect of sex; D, indicates a main effect of denervation; **, p < 0.01; ****, p < 0.0001; control n = 14–16, denervated n = 7–8