Table 1 List of antibodies used for western blotting
Protein | Concentration | Company | Catalogue Number |
|---|---|---|---|
ATG7 | 1:1000 | Sigma-Aldrich | A2856 |
Beclin1 | 1:1000 | Cell Signaling Technology | 3738Â S |
Cathepsin B | 1:1000 | Cell Signaling Technology | 31,718Â S |
Cathepsin D | 1:1000 | Santa Cruz Biotechnology | SC-377,299 |
LC3 | 1:500 | Cell Signaling Technology | 4108Â S |
MCOLN1 | 1:1000 | Invitrogen | PA1-46474 |
Parkin | 1:1000 | Cell Signaling Technology | 4211Â S |
TFEB | 1:500 | MyBioSource | MBS120432 |
TFE3 | 1:500 | Sigma-Aldrich | HPA023881 |
v-ATPase | 1:1000 | Santa Cruz Biotechnology | SC-55,544 |
HRP-linked Anti-mouse | As per manufacturer’s suggestions | Cell Signaling Technology | 7074 S |
HRP-linked Anti-rabbit | As per manufacturer’s suggestions | Cell Signaling Technology | 7076 S |