Figure 5

Effect of EK mutation on growth signaling pathways. (A) Representative anti-puromycin western blot for soleus muscle homogenates from EK and WT mice treated with insulin 25 minutes after puromycin. Also shown is a protein stain as a loading control. (B) Representative anti-puromycin western blot for EDL muscle homogenates from EK and WT mice treated with insulin 25 minutes after puromycin. Also shown is a protein stain as a loading control. (C) Analysis of anti-puromycin/protein in the soleus and EDL of saline and insulin-treated EK and WT mice. (D) Western blot for protein involved in protein synthesis as %WT average for each western blot using homogenates from the soleus and EDL muscles from mice treated with saline or insulin. (E and F) Analysis of indicated phospho protein to dephosphorylated proteins (plotted as %WT average) in soleus and EDL. (G and H) Analysis of indicated phospho protein to dephosphorylated protein (plotted as %WT average) in soleus and EDL. (I) Pathways altered by the EK mutation alter protein synthesis. Values are shown as mean ± SEM. *P <0.05 and **P <0.01.