- Open Access
Ca2+ permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca2+ signaling to sustain muscle function
Skeletal Muscle volume 5, Article number: 4 (2015)
Ca2+ influx through CaV1.1 is not required for skeletal muscle excitation-contraction coupling, but whether Ca2+ permeation through CaV1.1 during sustained muscle activity plays a functional role in mammalian skeletal muscle has not been assessed.
We generated a mouse with a Ca2+ binding and/or permeation defect in the voltage-dependent Ca2+ channel, CaV1.1, and used Ca2+ imaging, western blotting, immunohistochemistry, proximity ligation assays, SUnSET analysis of protein synthesis, and Ca2+ imaging techniques to define pathways modulated by Ca2+ binding and/or permeation of CaV1.1. We also assessed fiber type distributions, cross-sectional area, and force frequency and fatigue in isolated muscles.
Using mice with a pore mutation in CaV1.1 required for Ca2+ binding and/or permeation (E1014K, EK), we demonstrate that CaV1.1 opening is coupled to CaMKII activation and refilling of sarcoplasmic reticulum Ca2+ stores during sustained activity. Decreases in these Ca2+-dependent enzyme activities alter downstream signaling pathways (Ras/Erk/mTORC1) that lead to decreased muscle protein synthesis. The physiological consequences of the permeation and/or Ca2+ binding defect in CaV1.1 are increased fatigue, decreased fiber size, and increased Type IIb fibers.
While not essential for excitation-contraction coupling, Ca2+ binding and/or permeation via the CaV1.1 pore plays an important modulatory role in muscle performance.
Excitation-contraction coupling (ECC) in skeletal muscle involves a mechanical interaction between the L-type voltage-dependent Ca2+ channel (CaV1.1) and the sarcoplasmic reticulum (SR) Ca2+ release channel (the ryanodine receptor, RyR1). Although Ca2+ entry through CaV1.1 is not required for skeletal muscle ECC , CaV1.1 opens after the initial voltage-gated release event to allow Ca2+ to both bind and permeate the channel pore . Like other voltage-dependent Ca2+ channels, CaV1.1 undergoes multiple conformational changes driven by membrane depolarization and/or Ca2+ movement (and/or binding) through the channel [3,4]. The opening of CaV1.1 actually occurs after the opening of RyR1 , and CaV1.1 has been suggested to facilitate RyR1 closing . Although there is only limited understanding of the role of Ca2+ permeation via CaV1.1, this influx has been suggested to regulate clustering of nicotinic acetylcholine receptors at the neuromuscular junction [7-9] and muscle plasticity .
Alterations in Ca2+ binding and permeation through CaV1.1 contribute to the pathological development of several muscle diseases [11-14]. Mutations in both RyR1 and CaV1.1 are associated with malignant hyperthermia (MH) in humans. The CaV1.1 mutations either enhance Ca2+ influx, increase the Ca2+ sensitivity of RyR1, and/or destabilize a closed state of RyR1. At least one of these MH mutations (R174W) ablates the L-type current and increases the sensitivity of RyR1 to caffeine, but does not alter ECC, demonstrating that the disease mechanism involves a distinct role for CaV1.1 . Together, these findings suggest a more extensive role for Ca2+ permeation via CaV1.1 in muscle function than what is presently understood.
In skeletal muscle, as in most cells, Ca2+ regulates multiple signaling pathways depending on the amplitude, frequency, duration, and location of the Ca2+ signal (reviewed by Tavi and Westerblad ). CaMKII is a major integrator of local Ca2+ signals that exhibits a privileged interaction with L-type channels (CaV1.x) due to its ability to cluster nearby and, upon Ca2+ binding, to interact with CaV1.x channels . CaV1.x channels prevail over other Ca2+ influx channels with respect to transducing excitation-transcription coupling (ETC) [18,19]. Hudmon et al.  demonstrated that CaMKII tethers to CaV1.2 and phosphorylates the carboxy-terminal tail of the α1 subunit to increase Ca2+ influx. The interaction of CaMKII with CaV1.2, in turn, facilitates CaMKII auto-phosphorylation.
To directly delineate the role of Ca2+ permeation through CaV1.1 in skeletal muscle function, we created a mouse with a Ca2+ binding and permeation defect in CaV1.1. We demonstrate that this CaV1.1-mediated pathway utilizes the flexibility of Ca2+ signaling (location, frequency, duration, and amplitude) to regulate CaMKII and calcineurin activation, thereby enhancing SR Ca2+ store refilling and protein synthesis to modulate fatigue susceptibility, muscle size, and fiber type distribution.
N-benzyl-p-toluene sulfonamide (BTS) was purchased from Tocris Biosciences (Bristol/UK, England), latrunculin A from AdipoGen (San Diego/California, United States), and 4-chloro-m-cresol (4CmC) from Pfaltz & Bauer (West Chester/Pennsylvania, United States). The calcium dyes fura-2 AM and mag-fluo-4 AM were purchased from Invitrogen (Grand Island/New York, United States). Molecular weight marker for western blotting was purchased from GenDEPOT (Barker/TX, United States). Every other reagent including Insulin, KN-92, KN-93, and the myristoylated autocamtide 2-related inhibitory peptide (AIP) were purchased from Sigma-Aldrich (St. Louis, United States).
In this study, male mice between six and 12 weeks old were used (The mice were generated by the Hamilton lab and backcrossed with the C57BL/6 J mice obtained from Jackson’s Laboratory (Bar Harbor/Maine, United States)), unless otherwise indicated. All mice were housed at room temperature with a 12:12 hour light-dark cycle and provided with food and water ad libitum. All procedures were approved by the Animal Care Committees at Baylor College of Medicine (Texas, United States) and the University of Rochester (New York, United States).
Creation of E1014K mice
The EK mutation was engineered on a genomic fragment of about 500 bp containing the exon-encoding residue E1014. A tetracycline resistance gene cassette (tet, for bacteria selection) and a neomycin cassette (neo, for ES cell selection) were inserted into the middle of the engineered fragment, flanked by about 250 bp homologies to CaV1.1. This selection marker-containing fragment was used to isolate a larger CaV1.1 genomic clone from a mouse 129 phage library via homologous recombination in Escherichia coli . Several clones were isolated and we chose one with an appropriate length of homologies on each side of the two cassettes for electroporation into AB2.2 ES from the 129SvEv cells. Recombinant ES clones were identified using southern blot analysis, and one of the clones was injected into blastocysts derived from C57BL/6 J mice to produce chimeras. The targeted allele was germ-line transmitted and the two selection cassettes were removed through crosses with Meox2-Cre mice .
To expedite the transfer of the E1014K mutation from the 129SvEv mouse sub-strain background to a congenic C57BL/6 J background, speed congenics were used, in addition to conventional backcrossing. We identified 92 microsatellites of maximal base-pair length disparity between the 129SvEv and C57BL/6 J strains. These microsatellites were chosen to representatively span the entire genome and show distinct electrophoretic separation. Primer pairs were selected to PCR amplify the chosen microsatellites to be resolved by electrophoresis in Spreadex EL300 Wide Mini Gels (Elchrom Scientific, Cham, Switzerland). Finally, we compared 129SvEv and C57BL/6 J DNA standards to DNA from our backcrossed mice, and selected mice with the most sequence homology to C57BL/6 J DNA to be used in the next backcross. This method both ensured congenicity between the wild-type (WT) and EK mutant mice and provided a means to more quickly begin experimentation.
Isolation of flexor digitorum brevis muscle fibers
Skeletal muscle fibers were isolated from the flexor digitorum brevis (FDB) muscle obtained from WT and EK mice as described .
Whole-cell patch clamp recordings of CaV1.1 currents
The whole-cell patch clamp technique was used to assess CaV1.1 currents (ICa) in FDB fibers isolated from WT and EK mice. FDB fibers were bathed in an external recording solution containing (in mM): 157 TEA-methanesulfonate, 2 CaCl2, 10 HEPES, 0.5 anthracene-9-carboxylic acid (9-AC), and 0.1 BTS, at pH 7.4, adjusted with TEA-OH. The patch pipette internal solution contained (in mM): 140 Cs-methanesulfonate, 10 HEPES, 20 Na-EGTA, and 4 MgCl2, at pH 7.4, adjusted with CsOH. All reagents here were purchased from Sigma Aldrich (St. Louis/Missouri, United States). The patch pipette resistance when placed in the external solution was between 0.6 and 1.0 Mohm. Fibers were voltage-clamped at a holding potential of −80 mV. Series resistance was compensated up to 80%. Data were sampled every 120 μs and filtered using a low pass Bessel filter (Axon Instrument, Jakarta/Selatan, Indonesia) with a 2 kHz cut-off frequency. ICa was activated by 200 ms depolarizing pulses ranging from −40 mV to +60 mV in 10 mV increments delivered every 10 seconds. CaV1.1 current-voltage relationships (ICa-V) were obtained from peak currents measured during each depolarization normalized to cell capacitance and plotted against the corresponding test potential. ICa-V data were then fitted by the following modified Boltzman equation:
where G max is the maximal L-channel conductance, V is test potential, V rev is the L-channel reversal potential, V 0.5 is the potential for half-maximal activation of G max , and k g is a slope factor.
CaV1.1 currents were analyzed using Igor Pro 6 (Lake Oswego, Oregon, United States) and Clampfit 9 (Sunnyvale, California, United States) software.
Measurements of electrically-evoked Ca2+ release in flexor digitorum brevis muscle fibers stimulated during a single twitch
Acutely isolated FDB fibers were loaded for 20 minutes at room temperature with 4 μM mag-fluo-4 AM in a Kreb’s Ringer solution containing (in mM): 146 NaCl, 5 KCl, 2 CaCl2, 1 MgCl2, and 10 HEPES, at pH 7.4. Fibers were then washed and incubated for 20 minutes in dye-free Ringer’s solution supplemented with 20 μM BTS, a skeletal muscle myosin inhibitor, to block contraction. Mag-fluo-4 AM-loaded FDB fibers were excited at 480 ± 15 nm and fluorescence emission detected at 535 ± 20 nm was collected at 10 kHz using a photomultiplier system. Electrical field stimulation (8 V, 1 ms, and 10 stimuli at 1 Hz) was elicited using a glass electrode placed adjacent to the cell of interest. Peak changes in mag-fluo-4 fluorescence for all 10 stimuli were measured as (Fmax-F0)/F0 and then averaged to generate a single peak value for each fiber. The rate of mag-fluo-4 fluorescence decay for the second, third, and fourth twitches for each fiber was fitted to a first order exponential function and the resulting amplitude and tau values were averaged.
Mn2+ quench measurements
Mn2+ quench of fura-2 emission was measured in myotubes loaded with 5 μM fura-2 AM for 1 h at 37°C in Kreb’s Ringer solution. Briefly, prior to Mn2+ quench measurements, myotubes (primary cultured cells from muscle of mice) were treated with 100 μM ryanodine to block RyR1-mediated Ca2+ release during subsequent KCl application. Fura-2-loaded myotubes were excited at the experimentally determined fura-2 isosbestic point (362 nm) and emission monitored at 510 nm during perfusion of 50 mM KCl in the presence of 0.5 mM Mn2+. Maximum rates of fura-2 quench during KCl application were determined and evaluated for statistical significance.
Calcium imaging in confocal line scan mode in flexor digitorum brevis muscle fibers stimulated with a single 50 Hz train
To monitor Ca2+ release during electrical stimulation, FDBs fiber were loaded with 5 μM of mag-fluo-4 AM for 30 minutes at room temperature in the presence of 20 μM of BTS. Loaded FDBs were placed on the stage of a confocal microscope with an adapted perfusion system (tyrode with 20 μM BTS at 0.5 mL/min) and imaged in line scan mode using the 20x objective (EC Plan-Neofluar) mounted in the confocal microscope (Zeiss LSM 510 meta, California, United States), one line was acquired every 1.15 milliseconds (3.66 μsec/pixel time) using the 488 nm excitation laser and the LP 505 emission filter (Zeiss, California, United States). FDBs were stimulated with 50 square electrical pulses (200 μsec duration) at 50 Hz and the produced florescence transients was normalized (F/F0) and plotted.
Measurement of Ca2+ transients during repetitive stimulation with 100 Hz trains
Isolated FDB fibers were loaded with 5 μM of mag-fluo-4-AM for 30 minutes at room temperature, followed by washout with fresh DMEM (Life technologies, NY, United States). Electrical stimulation was performed using two platinum wires placed at each side of the fiber oriented longitudinally and fatigue was induced with uninterrupted application of electrical trains (100 Hz, 250 ms, every 1.5 seconds; 0.17 duty cycle) for 300 seconds. For evaluation of RyR1-releasable SR Ca2+ store content, 1 mM of 4CmC was perfused at 3.25 ml/min, applied after 60 trains of electrical stimulation. Mag-fluo-4 fluorescence was collected at 20 Hz. Data were collected and analyzed using Metafluor version 6.2 software (Molecular Devices, California, United States).
Muscles were homogenized and lysed in ice-cold RIPA buffer consisting of (mM): 25 Tris pH 7.6, 150 NaCl, 1 Na3VO4, 10 NaPyroPO4, 10 β-glycerophosphate, 10 NaF, PMSF, protease inhibitor cocktail (Santa Cruz), 1% NP40, 1% sodium deoxycholate, and 0.1% SDS (Every reagents came from Sigma Aldrich, St. Louis, United States). Equal amounts of total protein from whole muscle lysates were resolved by electrophoresis, transferred to PVDF (Millipore, Billercia, United States) membrane and western blot analyses were performed using antibodies shown in Additional file 1: Table S1. LI-COR IRDye™ infrared dyes were used as secondary antibodies and immunoreactive bands were visualized using the Odyssey Infrared Imaging System (LI-COR) (LI-COR Inc, Lincoln, United States). To allow the use of data from multiple western blots, the fluorescent band intensity of each band within a single western was first normalized to GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) as a loading control and then calculated as %WT average from that specific western blot. Data were then pooled to give %WT ± SEM.
Co-localization studies and single fiber immunocytochemistry
Single FDB fibers plated on glass slides were fixed with 2% paraformaldehyde in 0.1 M phosphate buffer (PB) (21.6 mM Na2HPO4 and 81.4 mM NaH2PO4, pH7.2) for one hour at room temperature, washed twice with phosphate buffered saline (PBS) (3.8 mM NaH2PO4, 16.2 mM Na2HPO4, 150 mM NaCl, pH 7.4), permeabilized, and blocked in PBS containing 0.5% Triton X-100 and 5% BSA overnight at 4°C (All reagents come from Sigma Aldrich as described in Materials). Primary antibodies diluted in PBST (PBS containing 0.5% TX-100) were added to slides and incubated overnight at 4°C. After washing twice with PBS, Alexa-fluor conjugated antibodies were added. Fibers were washed three times with PBS for 10 minutes each and mounted in Fluoromount-G (SouthernBiotech, Birmingham, United States). Fibers were imaged using a Zeiss LSM 510 META confocal microscope with a 100x/1.30NA oil lens, HeNe 543 nm laser, and Argon 458,477,488,514 laser (Zeiss, California, United States).
Proximity ligation assay
We used proximity ligation assays (PLAs) to identify proteins that are within 40 Å of each other. PLA was performed on single FDB fibers plated on glass disks. Fibers were kept at 37°C in a 95% O2-5% CO2 incubator in DMEM solution supplemented with 10% FBS. Fibers were then fixed with 2% paraformaldehyde in 0.1 M PB and incubated with the primary antibodies. The PLA was performed with the Duolink kit (Olink Biosciences, Uppsala, Sweden) according to the instruction of the user manual using an anti-goat MINUS PLA and anti-rabbit PLUS PLA probes and the orange detection reagent (Cy3) (Olink Biosciences, Uppsala, Sweden). Fibers were imaged with confocal microscopy (Zeiss LSM 510 META, with a 100x/1.30NA oil lens and HeNe 543 nm laser). For analysis, Z stacks were projected and saved as a single image. The positive spots were counted with Image J (8-bit images filtered with a Gaussian Blur filter (Rasband, W.S Image J, U.S. National Institutes of Health, Bethesda, Maryland, United States), σ = 1, and same threshold per set adjusted at 15-30). The number of spots counted was normalized to the area of the fiber estimated from the width and length of the fiber in the image. For each set of experiments, the average counts of WT fibers (control) were set to 100% and the number of spots in each experimental condition was calculated as percentage change.
Eight-week-old WT and EK mice were fasted for 12 hours and then given an intraperitoneal injection of insulin (1 U/kg) diluted in saline. Control mice were injected with saline. After 7.5 minutes mice were sacrificed and muscles (soleus and EDL (Extensor Digitorum Longus)) were isolated, frozen in liquid nitrogen, and stored at −80°C until use. Muscle levels of pAkt1/2 and pGSK3β in the presence and absence of insulin were measured as described in Butler et al. .
Detection of puromycin-labelled proteins
For measurement of protein synthesis we used an in vivo SUnSET technique [24,25]. Briefly, mice (13 weeks of age) were food deprived for eight hours. Propofol (18 μl/g) (Abbott Laboratories, North Chicago, United States) was administered via an intraperitoneal injection 15 minutes before the puromycin injection. The mice were then given an intraperitoneal injection of puromycin (0.04 μmol/g BW) and sacrificed 35 minutes later. At 10 minutes before sacrificing, insulin or saline (control) was administered via intraperitoneal injection. Muscles were isolated, homogenized, and prepared for western blotting with anti-puromycin antibody. For normalization to total protein, the same western blots were stained with Swift Membrane Stain™ kit (G-Biosciences, St. Louis, United States).
Ras activity was measured using a Ras activity assay kit (Cytoskeleton, Denver, United States). Briefly, muscle was lysed in buffer and protein concentration was measured. Raf-Ras binding domain (RBD) beads (50 μl) were added to the muscle lysates (total 500 μl of 2 mg/ml lysates) and the mixture was incubated at 4°C on a rotator for one hour. After incubation, Raf-RBD beads were pelleted by centrifugation at 5,000 × g at 4°C for one minute and washed with wash buffer. The bound active Ras was eluted in the two × sample buffer by boiling for three minutes. Eluted protein was run on 12% gel, transferred to PVDF membrane, and immunoblotted with Ras-specific antibody. The westerns were normalized to the amount of GAPDH in the muscle lysates (60 μg).
Sarco/endoplasmic reticulum Ca2+-ATPase activity
Electrical stimulation of isolated muscle for signaling changes
To assess stimulation-induced changes in signaling pathways we used the method of Sakamoto et al. . Intact soleus and EDL muscles were removed and suspended between a force transducer and stationary anchor within a test chamber filled with warmed (35°C) Kreb’s Ringer solution (KRS) oxygenated with a 95/5% mixture of O2/CO2, as above. After a 30 minute resting equilibration period, muscles to be stimulated underwent a fatigue protocol (100 Hz, 200 ms train duration, one second intervals) for five minutes per muscle. At the completion of the stimulation protocol, rested and stimulated muscles were immediately frozen in liquid N2 and stored at −80°C. For muscles treated with KN-93, the drug was added to the chamber at the start of the 30 minutes equilibration period, at a final concentration of 5 μM.
Muscle force frequency and fatigue
Intact soleus and EDL muscles were removed and immediately immersed in incubation medium comprised of KRS (oxygenated with a 95/5% mixture of O2/CO2. Muscles were tied with sutures at the musculotendinous junction and suspended between a force transducer and stationary anchor within a test chamber filled with warmed (35°C), oxygenated incubation medium. After a 20 minutes rest to allow mounted muscles to equilibrate, muscle optimal length (l o ) was determined via single-twitch force generation measurements. Next, force frequency measurements were obtained at l o using frequencies from 15 to 300 Hz at 200 ms/train followed by a fatigue protocol performed over five minutes per muscle. The specific fatigue protocol for each muscle used was for the soleus: 15 Hz, 200 ms duration, one second intervals) and for EDL: 60 Hz, 200 ms duration, one second intervals. Muscle stimulation occurred within the test chamber using platinum electrodes attached to a Grass S48 stimulator and recorded within Chart5 (version 5.2) software (eDAQ Inc, Colorado Springs, United States).
Fibertyping with cryosections and immunostaining
Skeletal muscles (soleus and EDL) were dissected, embedded in OCT compound (Tissue-Tek, Torrance, United States), and frozen in 2-methylbutane (Sigma Aldrich, St. Louis, United States) precooled in liquid nitrogen. The frozen muscles were sectioned with 10-μm thickness using a SHANDON cryostat microtome (Thermo Electron Corporation, Madison, United States). Immunofluorescent staining was performed using specific antibodies against myosin heavy chain I (MHCI), IIa (MHCIIa), and IIb (MHCIIb) (DSHB, Iowa City, USA). Briefly, sections were rehydrated with PBS for 10 minutes, followed by incubation at 4°C overnight with MHCI (BA-F8, IgG2b), MHCIIa (SC-71, IgG1), and/or MHCIIb (BF-F3, IgGM) antibodies diluted 1:50 in PBS. After washing with PBS, muscle sections were incubated at room temperature for 90 minutes with isotype-specific AlexaFluor-594-conjugated goat anti-mouse IgG2b, AlexaFluor-488-conjugated goat anti-mouse IgG1, and AlexaFluor-594-conjugated goat anti-mouse IgGM secondary antibodies diluted 1:200. After three consecutive washes with PBS, muscle slides were mounted with VECTASHIELD mounting media (Vector Laboratories, Burlingame, United States). Images were taken under a fluorescence microscope (Olympus America, Center Valley, United States). The relative numbers of the different fiber types were quantified and normalized by the total number of muscle fibers per field.
Fiber cross-sectional area
For cross-sectional area (CSA) calculations, 10-μm-thick frozen sections were obtained from the mid-belly area of the soleus and EDL muscles. Sections were immunostained for fiber type as described in the previous section, imaged at 10x magnification through an Olympus DP70 camera (Olympus America, Center Valley, Pennsylvania, United States), and saved in .tif format. Saved images were then imported into Photoshop CSE version 10.0 (Adobe Systems, San Jose, California, United States) for analysis. First, measurement scale was established by tracing a within-image scale bar (μm). Next, myofiber CSA was measured by tracing the external border of individual myofibers using the Magnetic Lasso tool. Myofibers exhibiting evidence of tears or processing artifacts were excluded from the analysis. Recorded measurements were then exported into Excel for analysis, with resulting CSA values reported in μm2.
We performed a statistical analyses of two groups using the Student’s t-test. P <0.05 was considered to be statistically significant. *P <0.05, **P <0.01, and ***P <0.001 were used to indicate statistical significance.
Creation of EK mice
To explore the role of Ca2+ influx via CaV1.1, we created mice with a knock-in mutation (E1014K or EK) in the pore region of CaV1.1. The mutation of a glutamate to lysine residue in the repeat III pore region of CaV1.x abolishes both Ca2+ binding to this site  and divalent permeation through the channel without altering ECC . The targeting construct and data confirming the mutation are shown in Additional file 2: Figure S1. Speed congenics were used to obtain mice on a clean C57BL/6 J background. The mice were homozygous viable with no immediately obvious changes in phenotype (see below).
Effects of the EK mutation on myofibrillar Ca2+ handling
To confirm that the EK mutation abolishes Ca2+ influx through CaV1.1, we compared whole-cell Ca2+ currents (Additional file 3: Figure S2A and B) and average CaV1.1 current-voltage relationships (Figure 1A and B) in single FDB fibers from EK and WT mice. The EK mutation abolished inward L-type Ca2+ currents, but permitted outward monovalent Cs+ currents. The permeation of EK channels to monovalent cations (for example, Cs+ and Na+; ) is unlikely to alter resting membrane because the EK monovalent cation conductance is only activated at depolarized potentials. Also, since the activation kinetics of EK channels is slow [2,30] relative to the duration of the skeletal muscle action potential, Na+ flux through EK channels should be minimal, and thus unlikely to alter action potential properties or intracellular Na+ levels (approximately 10 mM). The magnitude and decay kinetics of voltage-gated Ca2+ transients (assessed using a low affinity Ca2+ indicator, mag-fluo-4 AM) elicited by a low frequency train of electrical stimulation (1 Hz) were not significantly different between FDB fibers from WT and EK mice (Figure 1C-F), demonstrating that ECC was not altered by the EK mutation.
We used a well-established Mn2+ quench of fura-2 fluorescence assay to determine the effect of the EK mutation on excitation-coupled Ca2+ entry (ECCE) . In WT myotubes, membrane depolarization induced by the addition of 50 mM KCl opens CaV1.1 channels, providing a pathway for Mn2+ entry to quench fura-2 fluorescence (Figure 1G, upper trace), with the maximum slope of KCl-induced Mn2+ quench directly reflecting Mn2+ entry through CaV1.1 channels (Figure 1H). Since the EK mutation in the CaV1.1 pore abolishes divalent ion permeation through CaV1.1 channels, KCl-induced Mn2+ quench (ECCE) is absent in myotubes from EK mice (Figure 1G, lower trace), consistent with ECCE reflecting Ca2+ entry via CaV1.1, as suggested by Bannister et al. . The EK fibers also showed a small but significant increase in baseline Ca2+ influx (Figure 1H).
While the data in Figure 1 indicate that ECC is not altered in EK fibers, we found significant differences in the amplitude of the Ca2+ transients during repetitive stimulation. During a single train of high frequency stimulation, the amplitude of the Ca2+ transients was lower in EK compared to WT fibers (Figure 2A and B). The EK mutation also increased the rate of decline of the amplitude of the Ca2+ transient during repetitive trains of high frequency stimulation (Figure 2C). After these repetitive trains of stimulation, readily releasable SR Ca2+ stores assessed with a maximal concentration (1 mM) of 4-chloro-m-cresol (4CmC) were significantly lower in EK compared to WT fibers (Figure 2D), suggesting greater Ca2+ store depletion in the EK fibers following repetitive stimulation. However, readily releasable SR Ca2+ stores were not different prior to electrical stimulation (data not shown).
We used a higher affinity Ca2+ indicator, fura-2 AM, to measure changes in cytosolic Ca2+ levels. Resting cytosolic Ca2+ levels did not differ between EK and WT fibers. However, following stimulation (same stimulation protocol as Figure 2C), cytosolic Ca2+ levels were higher in both EK and WT fibers (Figure 2E), but magnitude of the stimulation-induced increase in cytosolic Ca2+ was less in EK fibers. These findings suggest that Ca2+ permeation through CaV1.1 facilitates refilling of SR Ca2+ stores either directly or indirectly. The decreased Ca2+ transient amplitude during repetitive stimulation in EK fibers could be due to an decrease in Ca2+ influx via either store-operated or ECCE .
Alterations in Ca2+ handling in EK muscle could also arise from changes in Ca2+ handling proteins. However, we detected no differences in the expression levels of CaV1.1, RyR1, SERCA1, or SERCA2 (Figure 3A-E). We did, however, detect a decrease in calsequestrin (CSQ, antibody detects both CSQ1 and 2) in the soleus (Figure 3F). Sarcolipin levels were unchanged (Figure 3G). SERCA activity in muscle homogenates at a fixed Ca2+ concentration was not different between EK and WT muscle (Figure 3H). However, differences in cytoplasmic Ca2+ levels are likely to impact SERCA activity in intact fibers during repetitive stimulation.
Pleiotropic effects of the EK mutation on Ca2+-sensitive pathways
The decreased amplitude of the Ca2+ transient during repetitive stimulation in EK fibers is likely to impact multiple muscle Ca2+ signaling pathways. As shown in Figure 4A, KN-93 decreases the amplitude of the Ca2+ transient in WT but not EK fibers, suggesting a role for CaMKII. To evaluate the effects of the EK mutation on CaMKII, we first assessed the ratio of pT286-CaMKII to total CaMKII using western blotting. Auto-phosphorylation of CaMKII at T286 is associated with its constitutive activation . We found that the ratio of p-CaMKII to CaMKII was decreased in the soleus and EDL of EK mice (Figure 4B). Since CaMKII is a known integrator of Ca2+ signals and has an interaction with L-type channels in other tissues , we used immunocytochemistry and proximity ligation assays (PLA) to determine if CaMKII was located close to CaV1.1 in skeletal muscle FDB fibers. We found that a significant amount of CaMKII co-localizes with CaV1.1 in both WT and EK fibers by immunocytochemistry (Figure 4C-E) and by PLA (Figure 4F and G). As assessed in the PLA assay, the interaction of CaV1.1 and CaMKII was decreased in the EK compared to WT fibers under both resting and electrically stimulated conditions (Figure 4G). Thus, CaMKII is in close proximity to CaV1.1 and this interaction is decreased by the permeation defect in CaV1.1.
We next assessed the effects of inhibition of CaMKII in WT fibers on refilling of SR Ca2+ stores during repetitive stimulation. We used a specific CaMKII inhibitory peptide, AIP (myristoylated CaMKII auto-inhibitory peptide) , and found that CaMKII inhibition increased the rate of decline in the amplitude of the Ca2+ transients (Figure 4H,) and reduced post-stimulation 4CmC releasable Ca2+ stores (Figure 4I) in FDB fibers from WT mice. KN-93 (but not the inactive K-92 analog) also reduced the amplitude of the Ca2+ transients in WT fibers but had no effect in EK fibers (Additional file 3: Figure S2C and D and Figure 4H). These data suggest that SR Ca2+ store refilling during repetitive stimulation in WT fibers is enhanced by CaV1.1-mediated activation of CaMKII, but this pathway is prevented by the EK mutation in CaV1.1 (Figure 4J).
Ca2+ is required for mTORC1 activation of p70 ribosomal S6 kinase 1 (S6K1) [35-39]. To determine if the absence of Ca2+ permeation through CaV1.1 altered protein synthesis, we used the SUnSET technique  to assess protein synthesis in EK and WT muscle. Although we detected a small decrease in protein synthesis in the absence of insulin in the soleus, protein synthesis was significantly decreased in both the soleus and EDL muscle of EK mice treated with insulin compared to insulin-treated WT mice (Figure 5A-C). To further analyze protein synthesis pathways downregulated by the absence of Ca2+ permeation through CaV1.1, we examined the phosphorylation status of several key regulators of protein synthesis. We found that pS2448 mTOR, p-T37/T46-4EBP1/4EBP1, and p-S235/S236-S6/S6 were reduced in the soleus and EDL of insulin-treated EK mice compared to insulin-treated WT mice (Figure 5D-F). To identify upstream events that regulate protein synthesis, we examined the levels of p-S473-Akt/Akt (phosphorylated by mTORC2) , pT308-Akt/Akt (phosphorylated by PDK1), and pT202/Y204-ERK1/2 and found that all of these phosphorylation events were decreased in both the soleus and EDL of EK mice treated with insulin compared to the corresponding muscle of insulin-treated WT mice (Figure 5D, G, and H). Hence the muscle response to insulin is blunted in the EK mice due to decreased mTORC2 and PDK1 phosphorylation of Akt and decreased ERK1/2 activity (Figure 5I). There are multiple Ca2+ and/or CaMKII sensitive step(s) upstream of mTORC2, PDK1, and ERK signaling, especially in the steps that lead to Ras activation.
The data with and without insulin suggest that the effects of the EK mutation on insulin-stimulated protein synthesis involve both Akt phosphorylation (PDK1 and mTORC2) and ERK signaling. To assess Ras activation in EK and WT muscle, we pulled down activated Ras (GTP bound) with Raf-RBD-beads from soleus and EDL muscle and western blotted with RAS specific antibody to evaluate the amount of Ras that pulled down. As shown in Figure 6A and B, less GTP-bound Ras was pulled down in EK compared to WT muscle. To further assess the role of Ca2+ permeation and binding to CaV1.1 and activation of CaMKII in the regulation of these pathways, we isolated soleus and EDL muscle from WT and EK mice, electrically stimulated in the presence and absence of KN-93 (an inhibitor of CaMKII) and again analyzed the pathways described in Figure 5. Electrical stimulation increased p-CaMKII/GAPDH, p-Raf/Raf (a CaMKII target), p-ERK1/2/GAPDH, p-S6/S6, and p-4EBP1/4EBP1 (Figure 6C-L), and these increases were less in the presence of the CaMKII inhibitor, KN-93. With the exception of p-Raf/Raf, all of these changes were detected in both soleus and EDL. Phosphorylated Raf was detected only at very low levels in the soleus. In contrast to the findings in WT muscle, no increases in p-CaMKII/GAPDH, p-Raf/Raf (a CaMKII target ), p-ERK1/2/GAPDH, p-S6/S6, and p-4EBP1/4EBP1 were detected in the EK muscle subjected to electrical stimulation.
We also examined the phosphorylation of Akt at S473 and T308 and found that phosphorylation of Akt at these sites were not significantly increased by electrical stimulation (Additional file 3: Figure S2E-H), suggesting that the signaling in response to repetitive stimulation is primarily through Ras/Raf activation of ERK1/2. Given these findings, we propose that Ca2+ permeation though or binding to CaV1.1 activates CaMKII which, in turn, phosphorylates Raf to activate Ras/Raf and ERK1/2. ERK1/2 then activates mTORC1 (Figure 6M). This stimulation-dependent signaling pathway is strongly blunted in EK muscle.
Together, our data provide compelling evidence that the EK mutation in CaV1.1 causes significant disruption to CaMKII and Ca2+-sensitive pathways in skeletal muscle, resulting in decreased SR store refilling and a decline in protein synthesis.
Effects of EK mutation on muscle function
The observed changes in activity dependent Ca2+ handling, Ca2+-dependent signaling and protein synthesis in muscle suggest that the EK mutation should alter muscle function. Although the ability to generate force (normalized to cross-sectional area) was not different between muscles of young (between eight and 12 weeks of age) WT and EK mice (Figure 7A and B), both the soleus and the EDL muscles of young (between eight and 12 weeks of age) EK mice displayed small, but significant increases in the rate of fatigue (Figure 7C and D). However, older mice (over nine months) show significant decreases in force generation in the soleus (Figure 7E) and further increases in fatigue in the EDL (Figure 7F). Changes in muscle force and fatigue could arise from changes in fiber type distribution. We found that the fraction of fast twitch type IIb fibers increased and type IIx fibers decreased in both soleus and EDL muscle (Figure 7G-J), but the soleus also showed a decrease in Type 1 fibers (Figure 7I). A decrease in fiber CSAs in all fiber types in both soleus and EDL was also observed in EK compared to WT mice (Figure 7K and L). We also used a type IIb specific antibody to separately determine the CSA of the type IIb fibers in EDL muscles and found a significant (P <0.001) decrease of approximately 25% (CSAWT = 1,810 ± 12 μm2, n = 1112 fibers; CSAEK = 1,370 ± 14 μm2, n = 898 fibers, each from three different mice). However, we found no significant differences in grip strength, voluntary monitored wheel running, or endurance running in young (eight to 12 weeks of age) EK compared to WT mice, suggesting that the effects of the EK mutation on fiber type distribution, muscle contractility, and fatigue are relatively mild in young mice but increase in severity with age.
Although acute changes in depolarization-induced contraction in low Ca2+ and in the presence of dihydropyridines were previously reported , the role of Ca2+ permeation through CaV1.1 in skeletal muscle has largely been ignored because Ca2+ influx through this channel is not required for ECC [2,43]. We demonstrate that Ca2+ signaling in skeletal muscle is significantly altered by a permeation defect in CaV1.1 (E1014K, EK). This mutation resulted in concurrent inhibition of multiple Ca2+-sensitive pathways. These signaling changes translated to significant effects on muscle contractile function, providing compelling evidence that Ca2+ binding and/or permeation via CaV1.1 is important for maintaining optimal muscle physiology.
The question that arises is whether the amount of Ca2+ entering the muscle fiber through CaV1.1 is adequate to directly drive these changes in intracellular Ca2+ handling during repetitive stimulation. If so, previous studies have either underestimated the magnitude and relative contribution of Ca2+ influx through CaV1.1 during repetitive stimulation of muscle, or CaV1.1 Ca2+ binding and permeation activates a pathway (such as CaMKII) that promotes Ca2+ entry through a second Ca2+ influx channel (such as ECCE, SOCE, or TRP). Another possibility is that Ca2+ binding to the selectivity filter triggers a conformational change in CaV1.1 that drives autophosphorylation of CaMKII bound to the channel (such as the C-terminus). CaMKII, activated by this mechanism, would then modulate multiple Ca2+ handling and downstream signaling pathways (SR Ca2+ stores, Ras, and mTORC1). The activation of these pathways would then alter numerous aspects of muscle physiology including fatigue, muscle fiber CSA, type II fiber type specification, and protein synthesis. Indeed, we demonstrate that autophosphorylation of CaMKII is decreased in the muscle of EK mice that have undergone repetitive stimulation, a condition that significantly enhances p-CaMKII levels in WT muscle.
The activation of different Ca2+-sensitive signaling pathways depends on the location, frequency, amplitude, and duration of the Ca2+ signal (reviewed by Tavi and Westerblad ). Important signaling molecules activated by Ca2+ use different combinations of these mechanisms. We demonstrate that CaMKII is localized close to CaV1.1 and that the EK mutation decreases the proximity of CaMKII to CaV1.1. Ca2+ permeation through CaV1.1, together with CaMKII activation in WT fibers, slows the decline of the Ca2+ transient with repetitive stimulation by enhancing Ca2+ store refilling. This mechanism is deficient in fibers from EK mice. CaMKII activation is decreased when Ca2+ cannot permeate CaV1.1, suggesting that Ca2+ binding within and/or movement through the CaV1.1 pore is important for activation of CaMKII. Reciprocally, CaMKII appears to amplify Ca2+ influx (either through CaV1.1 or a closely associated CaMKII-sensitive Ca2+ entry channel), since Ca2+ store refilling is decreased by CaMKII inhibitors in WT, but not EK fibers. However, at this point it is unclear whether the observed reduction in activity-dependent SR Ca2+ store content in FDB fibers from EK mice is due to loss of CaV1.1 Ca2+ influx or a downstream effect of CaMKII activation on SR Ca2+ reuptake and/or store-operated Ca2+ entry.
One of the major findings in this study was a substantial decrease in insulin-mediated protein synthesis in EK muscle, as assessed by puromycin labelling and immunoblot analysis of the mTOR signaling pathway. The mTORC1 pathway activation in response to electrical stimulation is also decreased in EK compared to WT muscle. Decreased protein synthesis in response to growth factors such as insulin and repetitive stimulation may underlie the observed decrease in muscle fiber size in EK muscle. Activation of downstream substrates of mTORC1 was significantly decreased in EK muscle, as was activation of the upstream regulators of mTOR, Akt, and ERK1/2. We suggest that the CaV1.1/CaMKII-dependent event that modulates insulin-mediated protein synthesis is upstream of PDK1 and/or mTORC2 activation, since phosphorylation of both Akt T308 (a PDK1 target) and Akt-S473 (an mTORC2 target) [44,45] are decreased in EK muscle following insulin treatment. This hypothesis is supported by previous studies that have reported Ca2+-dependent regulation of the PI3K/Akt/mTOR and Ras/Raf/ERK pathways [35-39].
The phenotypic changes associated with the EK mutation do not arise from any obvious changes in the neuromuscular junction. Chen et al.  found that a decrease in Ca2+ influx associated with a deficiency in the β-subunit of CaV1.1 altered the formation of the neuromuscular junction. This may reflect a recently identified transcriptional role for the β1a-subunit , as opposed to its more conventional role in controlling CaV1.1 Ca2+ currents, since mice with the EK mutation in CaV1.1 lack Ca2+ currents but do not exhibit alterations in neuromuscular junction preformatting (Additional file 4: Figure S3). We also did not detect any changes in immune function (Additional file 5: Figure S4).
While the magnitude of Ca2+ permeation through CaV1.1 is small, there is a significant impact of this pathway on muscle function. Changes include increased fatigue, decreased muscle fiber diameter, increased type IIb fiber specification, and decreased protein synthesis. Thus, our results indicate that small changes in CaV1.1 Ca2+ permeation, and CaMKII activation are amplified by changes in the activity of multiple critical downstream signaling pathways that impact a broad range of skeletal muscle functions. We propose that Ca2+ permeation through CaV1.1, while not required for ECC, modulates multiple downstream Ca2+-sensitive signaling pathways to improve muscle function.
autocamtide 2-related inhibitory peptide
calmodulin-dependent protein kinase II
L-type voltage-dependent calcium release channel
excitation-coupled calcium entry
extensor digitorum longus
mice with a glutamate to lysine mutation in the position 1014 of CaV1.1
flexor digitorum brevis
- Fura 2 AM:
fura-2 acetoxymethyl ester
Glyceraldehyde 3-phosphate dehydrogenase
Krebs Ringer solution
myosin heavy chain I
myosin heavy chain IIa
myosin heavy chain IIb
optimal cutting temperature
indicates phospho form if in front of a protein name
phosphate buffered saline
proximity ligation assay
Ras binding domain
ryanodine receptor 1
Sodium dodecyl sulfate
Sarco/endoplasmic reticulum calcium ATPase
store operated calcium entry
Transient receptor potential
Chandler WK, Rakowski RF, Schneider MF. A non-linear voltage dependent charge movement in frog skeletal muscle. J Physiol. 1976;254:245–83.
Dirksen RT, Beam KG. Role of calcium permeation in dihydropyridine receptor function. insights into channel gating and excitation-contraction coupling. J Gen Physiol. 1999;114:393–403.
Catterall WA. Voltage-gated calcium channels. Cold Spring Harb Perspect Biol. 2011;3:a003947.
Stroffekova K. Ca2+/CaM-dependent inactivation of the skeletal muscle L-type Ca2+ channel (CaV1.1). Pflügers Archiv. Euro J Physiol. 2008;455:873–84.
Garcia J, Tanabe T, Beam KG. Relationship of calcium transients and charge movements in myotubes expressing skeletal and cardiac dihydropyridine receptors. J Gen Physiol. 1994;103:125–47.
Zhou J, Yi J, Royer L, Launikonis BS, González A, García J, et al. A probable role of dihydropyridine receptors in repression of Ca2+ sparks demonstrated in cultured mammalian muscle. Am J Physiol Cell Physiol. 2006;290:C539–53.
Rotzler S, Schramek H, Brenner HR. Metabolic stabilization of endplate acetylcholine receptors regulated by Ca2+ influx associated with muscle activity. Nature. 1991;348:337–9.
Caroni PS, Rotzler BJC, Brenner HR. Calcium influx and protein phosphorylation mediate the metabolic stabilization of synaptic acetylcholine receptors in muscle. J Neurosci. 1993;13:1315–25.
Chen F, Liu Y, Sugiura Y, Allen PD, Gregg RG, Lin W. Neuromuscular synaptic patterning requires the function of skeletal muscle dihydropyridine receptors. Nat Neurosci. 2011;14:570–7.
Jorquera G, Altamirano F, Contreras-Ferrat A, Almarza G, Buvinic S, Jacquemond V, et al. Cav1.1 controls frequency-dependent events regulating adult skeletal muscle plasticity. J Cell Sci. 2013;126:1189–98.
Vandebrouck C, Martin D, Colson-Van Schoor M, Debaix H, Gailly P. Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers. J Cell Biol. 2002;158:1089–96.
Friedrich O, von Wegner F, Chamberlain JS, Fink RH, Rohrbach P. L-type Ca2+ channel function is linked to dystrophin expression in mammalian muscle. PLoS One. 2008;3:e1762.
Tang ZZ, Yarotskyy V, Wei L, Sobczak K, Nakamori M, Eichinger K, et al. Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of Ca(V)1.1 calcium channel. Hum Mol Genet. 2012;21:1312–24.
Cherednichenko G, Ward CW, Feng W, Cabrales E, Michaelson L, Samso M, et al. Enhanced excitation-coupled calcium entry in myotubes expressing malignant hyperthermia mutation R163C is attenuated by dantrolene. Mol Pharmacol. 2008;73:1203–12.
Eltit JM, Bannister RA, Moua O, Altamirano F, Hopkins PM, Pessah IN, et al. Malignant hyperthermia susceptibility arising from altered resting coupling between the skeletal muscle L-type Ca2+ channel and the type 1 ryanodine receptor. Proc Natl Acad Sci U S A. 2012;109:7923–8.
Tavi P, Westerblad H. The role of in vivo Ca2+ signals acting on Ca2+-calmodulin-dependent proteins for skeletal muscle plasticity. J Physiol. 2011;589:5021–31.
Hudmon A, Schulman H, Kim J, Maltez JM, Tsien RW, Pitt GS. CaMKII tethers to L-type Ca2+ channels, establishing a local and dedicated integrator of Ca2+ signals for facilitation. J Cell Biol. 2005;171:537–47.
Abiria SA, Colbran RJ. CaMKII associates with CaV1.2 L-type calcium channels via selected beta subunits to enhance regulatory phosphorylation. J Neurochem. 2010;112:150–61.
Ma H, Cohen S, Li B, Tsien RW. Exploring the dominant role of Cav1 channels in signalling to the nucleus. Biosci Rep. 2012;33:97–101.
Zhang P, Li MZ, Elledge SJ. Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step. Nat Genet. 2002;30:31–9.
Tallquist MD, Soriano P. Epiblast-restricted Cre expression in MORE mice: a tool to distinguish embryonic vs. extra-embryonic gene function. Genesis. 2000;26:113–5.
Beam KG, Knudson CM. Calcium currents in embryonic and neonatal mammalian skeletal muscle. J Gen Physiol. 1988;91:781–98.
Butler M, McKay RA, Popoff IJ, Gaarde WA, Witchell D, Murray SF, et al. Specific inhibition of PTEN expression reverses hyperglycemia in diabetic mice. Diabetes. 2002;51:1028–34.
Goodman CA, Mabrey DM, Frey JW, Miu MH, Schmidt EK, Pierre P, et al. Novel insights into the regulation of skeletal muscle protein synthesis as revealed by a new nonradioactive in vivo technique. FASEB J. 2011;25:1028–39.
Schmidt EK, Clavarino G, Ceppi M, Pierre P. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009;6:275–7.
Schertzer JD, Green HJ, Fowles JR, Duhamel TA, Tupling AR. Effects of prolonged exercise and recovery on sarcoplasmic reticulum Ca2+ cycling properties in rat muscle homogenates. Acta Physiol Scand. 2004;180:195–208.
Gehrig SM, van der Poel C, Sayer TA, Schertzer JD, Henstridge DC, Church JE, et al. Hsp72 preserves muscle function and slows progression of severe muscular dystrophy. Nature. 2012;484:394–8.
Sakamoto K, Hirshman MF, Aschenbach WG, Goodyear LJ. Contraction regulation of Akt in rat skeletal muscle. J Biol Chem. 2002;277:11910–7.
Yang J, Ellinor PT, Sather WA, Zhang JF, Tsien RW. Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels. Nature. 1993;366:158–61.
Bannister RA, Beam KG. Properties of Na+ currents conducted by a skeletal muscle L-type Ca2+ channel pore mutant (SkEIIIK). Channels (Austin). 2011;5:262–8.
Cherednichenko G, Hurne AM, Fessenden JD, Lee EH, Allen PD, Beam KG, et al. Conformational activation of Ca2+ entry by depolarization of skeletal myotubes. Proc Natl Acad Sci U S A. 2004;101:15793–8.
Bannister RA, Pessah IN, Beam KG. The skeletal L-type Ca2+ current is a major contributor to excitation-coupled Ca2+ entry. J Gen Physiol. 2009;133:79–91.
Yang E, Schulman H. Structural examination of autoregulation of multifunctional calcium/calmodulin-dependent protein kinase II. J Biol Chem. 1999;274:26199–208.
Ishida A, Kameshita I, Okuno S, Kitani T, Fujisawa H. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995;212:806–12.
Mercan F, Lee H, Kolli S, Bennett AM. Novel role for SHP-2 in nutrient-responsive control of S6 kinase 1 signaling. Mol Cell Biol. 2013;33:293–306.
Graves LM, He Y, Lambert J, Hunter D, Li X, Earp HS. An intracellular calcium signal activates p70 but not p90 ribosomal S6 kinase in liver epithelial cells. J Biol Chem. 1997;272:1920–8.
Hannan KM, Thomas G, Pearson RB. Activation of S6K1 (p70 ribosomal protein S6 kinase 1) requires an initial calcium-dependent priming event involving formation of a high-molecular-mass signalling complex. Biochem J. 2003;370:469–77.
Gulati P, Gaspers LD, Dann SG, Joaquin M, Nobukuni T, Natt F, et al. Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34. Cell Metab. 2008;7:456–65.
Conus NM, Hemmings BA, Pearson RB. Differential regulation by calcium reveals distinct signaling requirements for the activation of Akt and p70S6k. J Biol Chem. 1998;273:4776–82.
Yang Q, Inoki K, Kim E, Guan KL. TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proc Natl Acad Sci U S A. 2006;103:6811–6.
Salzano M, Rusciano MR, Russo E, Bifulco M, Postiglione L, Vitale M. Calcium/calmodulin-dependent protein kinase II (CaMKII) phosphorylates Raf-1 at serine 338 and mediates Ras-stimulated Raf-1 activation. Cell Cycle. 2012;11:2100–6.
Dulhunty AF, Gage PW. Effects of extracellular calcium concentration and dihydropyridines on contraction in mammalian skeletal muscle’. J Physiol. 1988;399:63–80.
Armstrong CM, Bezanilla FM, Horowicz P. Twitches in the presence of ethylene glycol bis(-aminoethyl ether)-N, N’-tetracetic acid. Biochim Biophys Acta. 1972;267:605–8.
Alessi DR, James SR, Downes CP, Holmes AB, Gaffney PR, Reese CB, et al. Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. Curr Biol. 1997;7:261–9.
Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098–101.
Taylor J, Pereyra A, Zhang T, Messi ML, Wang ZM, Hereñú C, et al. The Cavβ1a subunit regulates gene expression and suppresses myogenin in muscle progenitor cells. J Cell Biol. 2014;205:826–46.
This work is supported by grants from the Muscular Dystrophy Association and NIH (AR053349 to SLH and RTD, AR041802 to SLH, AR060831 to VY, and NIH T32 HL007676 to MK, JO, and JAL), postdoctoral fellowship from the Mexican Council of Science and Technology (186607) to ADA, and a postdoctoral fellowship from the Swedish Research Council to JTL.
The authors declare that they have no competing interests.
All authors participated in the preparation of the manuscript. CSL designed experiments and performed all of the western blot analyses in Figure 3, the western blots and immunocytochemistry in Figure 4, the proximity ligation assays in Figure 4, generated and analyzed the data in Figures 5 and 6 (together with AH), and the fiber type analysis in Figure 7. ADA designed, generated, and analyzed the data in Figure 2 and panels G and H in Figure 4. VY generated the data in Figure 1A-F. AH designed and performed the experiments in Figure 6 and the experiments in Figure 7E-F. AL generated and analyzed the data in Figure 1G and H. MK generated and analyzed the force frequency and fatigue data in Figure 7A-F. DKG performed the SERCA assays, analyzed the PLA data, designed experiments, and helped write the paper. RAP generated and analyzed the data in Additional file 4: Figure S3. MWS generated and analyzed date for force frequency and fatigue in Figure 7. CL preformed experiments with myotubes that laid the ground work for the FDB data generated in Figure 3. III performed some of the experiments that were used in Figure 3. JL, KD, and TT performed the exercise studies on the EK mice. TT and KD handled all mouse genotyping and matings. GGR helped to design and supervise the force frequency and fatigue experiments. MED helped to design and supervise the confocal imaging. CB performed studies shown in Additional file 5: Figure S4 to demonstrate that immune function was not altered in these mice. PZ designed the targeting vectors for the creation of the EK mice. RTD helped to conceive the study, designed the Ca2+ imaging experiments, and supervised the Ca2+ imaging studies in Figure 1 and helped to write multiple drafts of the manuscript. SLH conceived the study, participated in the design of many of the experiments, supervised all data collection and analyses and wrote the paper. All authors read and approved the final manuscript.
CSL is an Instructor at the Department of Molecular Physiology and Biophysics at the Baylor College of Medicine (BCM). ADA is currently an Assistant Professor at the University of Colima in Mexico. VY is currently an Assistant Professor at the Department of Pharmacology and Physiology University of Rochester Medical Center. AH is a postdoctoral fellow at Baylor College of Medicine. MK is currently an Assistant Professor at the University of Houston. DKG is an Assistant Professor at the Department of Molecular Physiology and Biophysics at BCM. RAP is currently an Assistant Professor at the Department of Molecular Physiology and Biophysics at BCM. MWS is currently an Assistant Professor at MD Anderson Cancer Center. CL is currently an Assistant Professor at the School of Life Sciences, South China Normal University. GGR is an Assistant Professor at the Department of Molecular Physiology and Biophysics at BCM. CB is an Associate Professor at the Department of Molecular Physiology and Biophysics at BCM. MED and PZ are Professors at the Department of Molecular Physiology and Biophysics at BCM. RTD is a Professor at the Department of Pharmacology and Physiology University of Rochester Medical Center. SLH is Professor and Chair of the Department of Molecular Physiology and Biophysics at BCM.
Creation of EK mice.
Effects of electrical stimulation.
EK mutation does not alter neuromuscular junction (NMJ) pre-patterning (A-F).
Analysis of the immune phenotype did not identify any significant differences between EK and WT mice.
About this article
Cite this article
Lee, C.S., Dagnino-Acosta, A., Yarotskyy, V. et al. Ca2+ permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca2+ signaling to sustain muscle function. Skeletal Muscle 5, 4 (2015). https://doi.org/10.1186/s13395-014-0027-1
- CaM kinase II
- Fiber type
- Protein synthesis and Skeletal muscle