Protein kinase A

Protein kinase A (PKA), or cAMP-dependent protein kinase, was discovered over 40 years ago, the second protein kinase to be described [5]. It is involved in a multitude of cellular processes and has hundreds of substrates [6]. In its inactive form, it exists as a holoenzyme containing two catalytic and two regulatory subunits. Each regulatory subunit can bind cAMP, and binding triggers dissociation of the holoenzyme and the release/activation of the catalytic subunits. Elevation in intracellular cAMP levels is therefore the primary mechanism of activation for PKA.

PKA is required at multiple stages during myogenesis, but an initial requirement is found during embryogenesis and the formation of myogenic precursors (i.e. myoblasts) within the dermomyotome (Figure 2). PKA activity is required for the expression of Pax3, MyoD and Myf5 in cells of the dermomyotome that will transition to form the myotome proper [7]. This activity is initiated by the release of Wnt1 and Wnt7a from the dorsal neural tube and surface ectoderm, respectively, which activate heteromeric G proteins and adenylate cyclase to produce cAMP, activating PKA. As PKA has many substrates, the expression of these myogenic genes is likely accomplished through several different targets, but at least one of these is the transcription factor cAMP response-element binding protein (CREB). Phosphorylation of CREB by PKA allows it to initiate the transcription of genes that contain a CRE element, two of which are Pax3 and Myf5. Chen et al.[7] showed that phosphorylated CREB is present at high levels in cells of the dermomyotome that express Pax3, MyoD and Myf5 and that this phosphorylation is critical for the induction of these genes. What is interesting about PKA with regards to myogenesis is that although it triggers the expression of several myogenic transcription factors, it also acts to suppress their transcription activity. It has been shown that PKA can inhibit the activity of Myf5, MyoD, myogenin and MEF2D without affecting their ability to bind DNA [8–10]. In the case of the MRFs, this appears to occur via an intermediary mechanism as opposed to direct phosphorylation, but in the case of MEF2D it is direct.

It is not clear what happens at the onset of differentiation with regards to PKA, although previous results suggest that PKA activity ultimately drops as differentiation proceeds, at least in C2C12 cells [11]. Obviously, its repressive effect on the MRFs and MEF2D must be removed for differentiation to occur, and this could arise through a reduction in cAMP levels, but what happens to cAMP upon differentiation is uncertain. Different groups have reported conflicting results regarding cAMP levels and their effect on myoblast differentiation in secondary cell lines [9, 11–14]. What seems clear from a recent study utilizing primary myoblasts and C2C12 cells is that cAMP does not have an inhibitory effect on differentiation, but rather enhances both fusion- and differentiation-associated hypertrophy [15]. Although PKA activity would presumably be involved as a consequence of elevated cAMP, this was not convincingly shown, as the inhibitor used in that study (H89) is not absolutely specific to PKA. It was convincingly shown, however, that the appropriate localisation of PKA is critical for the positive myogenic effect of cAMP and that this appropriate localisation may be to lamellipodia. Early work on PKA and myoblast differentiation in L6 cells revealed that the establishment of appropriate levels of the regulatory and catalytic subunits of PKA is critical for differentiation [16, 17]. It may be that PKA activity and cAMP are inhibitory to differentiation when present in certain areas (the nucleus, for example) and positive when found in other areas (lamellipodia). The repressive effect that PKA has upon the MRFs and MEF2D could be removed by a change in localisation or increased nuclear association of the catalytic subunit with its regulatory subunits, and this change may go hand in hand with a positive effect of PKA elsewhere in the cell. Ultimately, more detailed work on the role of PKA and cAMP during myoblast differentiation needs to be done to resolve these issues.

Cyclin-dependent kinases

The cyclin-dependent kinases (CDKs) take their name from a catalytic dependence on the cyclin family of regulatory proteins. There are several cyclins and CDKs that collectively control cell cycle progression as well as other processes. The cyclins, and by extension the CDKs, can be divided into three major groups: the G₁ cyclins, which regulate progression through G₁ and entry into S phase; the mitotic cyclins, which regulate entry into mitosis; and the non-cell cycle cyclins, which have cell cycle-independent roles. This last group is not a typical classification, but we introduce it here because this group of cyclins/CDKs is important for myogenesis. The G₁ cyclins include cyclins D and E, and are responsible for activating CDK4 (cyclin D), CDK6 (cyclin D) and CDK2 (cyclins D and E). The mitotic cyclins A and B activate CDK2 (cyclin A) and CDK1 (cyclin B). Levels of these cyclins are regulated by intrinsic cell cycle-derived signals, with the exception of cyclin D, which is regulated largely by extrinsic signals such as growth factors [18]. The final group of cyclins that have prominent roles outside the cell cycle include p35 and cyclin T, which activate CDK5 and CDK9, respectively. p35 is technically not a cyclin family member, but it activates CDK5 in the same allosteric manner as cyclins activate their CDKs and so we include it here.

In all cell types, the G₁ and mitotic cyclins/CDKs regulate cell cycle progression and proliferation, and myoblasts are no different (Figure 2). One of the major mechanisms by which cell cycle progression is mediated is through CDK-dependent phosphorylation of the retinoblastoma protein (Rb). When phosphorylated, Rb is unable to bind and inhibit the E2F family of transcription factors, whose activities drive cell cycle progression. In proliferating myoblasts, the CDKs have an additional role in preventing precocious differentiation. Cyclin E/CDK2 and cyclin D/CDK4 can both block differentiation and the transcriptional activity of MyoD [19–24]. Cyclin E/CDK2 blocks MyoD-induced gene expression through the phosphorylation of Rb [22], preventing Rb from binding and transactivating MyoD [25], and triggering S phase entry instead of differentiation. Overexpression (or the natural accumulation in myoblasts) of MyoD is one well-known way to drive myogenic differentiation, even in nonmyogenic cell lines. Cyclin E/CDK2 can phosphorylate MyoD at serine 200 [26–28], which causes ubiquitination and degradation of this transcription factor during G₁[28, 29], preventing its accumulation and a commitment to differentiation. Phosphorylation of MyoD at S200 is common to other CDKs, such as the mitotic cyclin B/CDK1 [26], which may prevent inappropriate MyoD accumulation during mitosis. In contrast to CDK2, cyclin D/CDK4 blocks MyoD activity through an as yet unclear mechanism that may involve direct binding [22, 30, 31]. Cyclin D/CDK4 can also block the activity of myogenin and all MEF2 isoforms [31]. Not much is known about how this occurs, but inhibition of MEF2C by CDK4 prevents the association of MEF2 with its transcriptional coactivator, glucocorticoid receptor-interacting protein 1 (GRIP1) [31]. Whether CDK6 also plays a role in preventing differentiation is unknown, although the mechanisms by which the CDKs block differentiation are likely much more complex than what we present here.

For myoblasts to differentiate, the cell cycle must be exited and the restraints the CDKs place on differentiation must be removed. Differentiation cues, such as serum withdrawal or cell-cell contact in cultured cells, elicit several effects that ultimately cause a decrease in G₁ and mitotic CDK activity. The expression of cyclin D1, CDK1, CDK2 and CDK6 drop with differentiation, while cyclins A, B and E may also decrease [19–21, 23, 26, 32–41]. At the same time as the expression levels of these proteins decline, there is an increase in the levels of the two families of CDK inhibitors (CKIs): the in hibitor of CDK4 family (INK4) and the c yclin-dependent kinase-i nteracting p rotein/k inase-i nhibitory p rotein family (CIP/KIP). The INK4 family includes p15, p16, p18 and p19, and, despite the family name, these members also inhibit CDK6. The CIP/KIP members include p21, p27 and p57, and these members inhibit all G₁ CDKs. There is substantial evidence demonstrating the importance of the CKIs for myoblast differentiation in vitro and in vivo. The expression levels of p16, p18, p19, p21, p27 and p57 all increase with differentiation [27, 33, 35, 38, 39, 41–46], there is a sharp increase in p27 levels in the myotome at the onset of development [47], and mice lacking p21 and p57 form defective muscles [48]. When myoblasts are cued to differentiate, the CKIs bind and inhibit the G₁ and mitotic CDKs [23, 33, 35, 36, 39, 41], and do so throughout differentiation and even in adult tissue, which is important as not all cell cycle CDKs are lost with differentiation [20, 23, 27, 35–38]. Unlike the other cyclins mentioned, cyclin D3 levels increase with differentiation, during which process this cyclin interacts strongly with CDK2 and CDK4 [19–21, 36, 37, 39, 41, 49]. However, CDK-containing cyclin D3 complexes lack activity, suggesting that cyclin D3 may fulfil a necessary role as part of an inhibitory complex during differentiation. The end result of these changes in protein expression, whether it be cyclins, CDKs or CKIs, is a net loss of cell cycle CDK activity [26, 32, 33, 35, 37–39], hypophosphorylation of Rb [34, 36, 38], cell cycle exit, accumulation of MyoD [27], and leave for myoblasts to differentiate.

Once the cell cycle CDKs have been effectively silenced and the cell cycle exited, the non-cell cycle CDKs are important for promoting and establishing differentiation (Figure 3). These include CDK5 and CDK9, which are not inhibited by the CKIs discussed above. The expression of the CDK5-activating protein p35 is induced with myoblast differentiation and during muscle regeneration, the more stable and active calpain cleavage product of p35 (p25) increases as differentiation progresses, and the activity of CDK5 subsequently increases [50–53]. Dominant-negative CDK5 that lacks activity inhibits both differentiation and fusion [50, 51, 54], although the mechanisms by which CDK5 activity promotes these processes are not clear. In myoblasts, CDK5 can interact with, phosphorylate and regulate nestin [51], a negative regulator of differentiation, while nestin in turn can feed back and control CDK5 activity by preventing the processing of p35 into p25 [54]. Through a bidirectional relationship, CDK5 and nestin appear to control the rate at which myoblast differentiation occurs. Like CDK5, the activity of CDK9 also increases with differentiation, and this activity is critical for both in vitro differentiation and in vivo regeneration following injury [55–57]. Overexpression of it (or its activating cyclin T2) accelerates differentiation, at least in part through enhancing the activity of MyoD [55]. Cyclin T2/CDK9 can interact with and phosphorylate MyoD, although the consequences of phosphorylation are not known [55, 57]. The interaction between these components, however, is critical for MyoD to induce gene expression. The recruitment of cyclin T2/CDK9 by MyoD to muscle-specific loci is believed to result in the phosphorylation and activation of RNA polymerase II by CDK9, thereby inducing transcription of myogenic genes [56]. Needless to say, what is known about these non-cell cycle CDKs and their role in differentiation is very partial, but highlights how the CDK family of kinases regulates myogenesis in a number of ways.

Extracellular signal-regulated kinase (FGFR, Raf, MEK, RSK2)

Extracellular signal-regulated kinase (ERK) was first identified as an insulin-sensitive kinase that could phosphorylate the microtubule-associated protein 2, hence its original name 'MAP2 kinase' or 'MAP2K' [58]. It was later given the more general name 'ERK' [59], as its activity can be stimulated by a variety of growth factors/mitogens and it has many substrates in addition to MAP2. It is still generally known as 'MAPK', but with 'MAPK' now an acronym for 'mitogen-activated protein kinase'. There are several ERK isoforms, and there are other kinases that go by the name 'ERK', but generally, when used, the name refers to isoforms ERK1 and ERK2.

ERK1 and ERK2 (ERK1/2) belong to a well-defined pathway (Figure 2) that is activated by growth factor stimulation of a receptor tyrosine kinase, such as the binding of fibroblast growth factor (FGF) to its receptor (FGFR). Autophosphorylation of the growth factor receptor follows ligand binding and initiates the formation of an adaptor complex through Src homology 2 (SH2) domain-containing proteins such as GRB2. GRB2 interacts with the guanine-nucleotide exchange factor SOS, and localisation of these two proteins to the plasma membrane near the GTPase Ras allows SOS to catalyse GTP exchange and activation of Ras. GTP-bound Ras then binds and activates Raf, initiating the MAP kinase cascade. Raf is a MAP kinase kinase kinase (MAP3K) that phosphorylates and activates the dual-specificity MAP or ERK kinase (MEK), which in turn phosphorylates and activates ERK1/2.

Myoblasts/myocytes have a unique biphasic requirement for ERK activity. ERK1/2 is critical for growth factor-induced cellular proliferation, inhibitory to myoblast differentiation, and later required for myocyte fusion, or at least ERK2 appears critical to this last process. One previous publication also suggested that the Raf-MEK-ERK pathway might play a role in maintaining satellite cell quiescence [60], but further experimentation is required before this can be accepted. What is disappointing about the research that has been done on this pathway and its role during myogenesis, or rather on ERK's role in particular, is that virtually no ERK target phosphorylations have been studied or even identified. It is assumed that relevant substrates will be canonical ERK targets that have been studied in other cell types, but this has not been shown and there may very well be novel muscle-specific substrates as well. Research has instead focused on discovery and further description of the stages of myogenesis that ERK regulates, with some insight into secondary mechanisms, but almost nothing on direct substrates and their role in the myogenic process. With that in mind, we shall proceed with a discussion of what is known about the function of ERK and this pathway during myoblast proliferation and differentiation.

Evidence from primary cell cultures suggests a critical role for ERK in myoblast proliferation [61, 62], which is supported by extensive data from secondary cell lines. In myoblasts, ERK activity can be stimulated by a variety of growth factors. Serum, a complex mixture of mitogens, activates ERK [63–66], but FGF [64, 67–71], hepatocyte growth factor (HGF) [61], insulin-like growth factor (IGF) [66, 67, 70, 72–74], leukaemia-inhibitory factor (LIF) [75], and platelet-derived growth factor (PDGF) [73, 76] can do so in isolation. Not all of these growth factors elicit the same response from ERK, however. FGF, HGF and IGF activate ERK to induce or maintain proliferation [61, 67, 69–72, 77], while PDGF does not but can enhance survival [73, 76]. During proliferation, ERK activity prevents cell cycle exit during G₁[78], and FGF/ERK's role during myoblast proliferation may be to prevent cell cycle exit and promote entry into S phase [69, 79]. How ERK accomplishes these functions, and particularly how different responses are elicited from it by different growth factors, is unknown. Of the different ERK-inducing growth factors, FGF has been the best studied in the context of myoblast proliferation, and the signalling cascade that results from FGF stimulation is as described above [69–71, 80, 81], although it should be mentioned that FGF appears to affect proliferation by an additional ERK-independent pathway as well [69, 82].

Although almost nothing is known about how ERK positively affects myoblast proliferation, not much more is known about how it prevents premature differentiation, although it is clear that it does. ERK only mediates this effect for certain growth factors, however. IGF and FGF can both stimulate ERK activity, but once cells reach confluency in culture, IGF stimulation promotes differentiation [66, 72] while FGF stimulation prevents it [64, 68–70, 76, 83–89]. This is likely due to IGF's ability to induce other pathways in addition to that of ERK (see Akt section below), and demonstrates how the role that ERK activity is playing needs to be considered within the physiological context in which it occurs. In the context of FGF-induced activity, the Ras-Raf-MEK-ERK pathway is able to inhibit differentiation [62, 64, 70, 78, 81, 88–100] by preventing the nuclear accumulation of MEF2 [96], and preventing the expression of certain myogenic factors, including MyoD [85–87, 101–104], the CDK inhibitor p21 [94, 95] and other transcriptional regulatory proteins [105]. ERK's and FGF's ability to prevent myoblast differentiation is supported by the biochemical observation that during differentiation FGF receptors are lost [106, 107] and the activity of ERK decreases [52, 66, 81, 92, 95, 97, 108]. Again it appears that this critical role of ERK in blocking differentiation occurs specifically during G₁[84], possibly as an inhibitory cue that prevents the accumulation of proteins that would drive cells into a postmitotic phenotype. As mentioned, the substrates that ERK acts on to prevent myoblast differentiation are unknown.

ERK activity does initially decrease with myoblast differentiation, which is necessary for differentiating myoblasts to overcome the inhibitory effect that it has, but ERK's activity comes back on as differentiation proceeds [66, 92, 93, 95, 97, 104, 108–110]. ERK activity, and specifically that of the ERK2 isoform, is critical for myocyte fusion and survival (Figure 3) [77, 92, 93, 95, 104, 109]. ERK can phosphorylate and activate the 90-kDa ribosomal S6 kinase 2 (RSK2), which positively regulates myocyte fusion through phosphorylation and transcriptional activation of nuclear factor of activated T cell 3 (NFAT3) [111]. ERK activity also stimulates the transcriptional activity of MyoD by an as yet to be described mechanism [104], and, contrary to ERK's role in myoblasts, it now enhances the expression of p21 [95, 110]. There may be uncoupling of the Raf-MEK-ERK pathway during myocyte fusion as there are contradictory data on the function of Raf, with different reports describing both positive and negative roles for it [94, 98, 104, 110], although it is clear that both MEK and ERK play positive roles. Similarly, FGF is certainly inhibitory to fusion, and so the growth factor or mechanism stimulating ERK activity in myocytes is unknown and the pathway promoting this activity needs further elucidation.

p38α (TAK1, MKK3/6)

The p38 family of MAPKs are closely related to the ERK MAPKs discussed above, and take their rather unimaginative name from their apparent molecular weight. The α isoform of the p38 family was initially identified as an effector of the cellular stress response [112–115], but has also been shown to be critical for the differentiation of numerous cell types [116–121]. There are three other p38 isoforms, β, γ and δ, but only p38α appears uniformly critical for differentiation, with the other isoforms either unnecessary or with insufficient evidence supporting an essential role.

At the turn of the century, several groups reported a critical role for one of or both the p38α and β isoforms during myoblast differentiation [95, 97, 122–125]. It was found that p38 activity was induced during differentiation in culture and that inhibition of the α and β isoforms blocked the induction and/or activation of myogenic and muscle-specific genes, as well as prevented myocyte fusion. These studies were all performed with secondary cell lines (C2C12, L6E9, L8 and transformed 10T1/2), but in vivo work has confirmed that p38 activity is indeed critical for myoblast differentiation. During embryonic development, p38 activity is induced in somites, and inhibition does not affect the myogenic commitment of cells but does block the induction of the myotomal muscle marker myosin light chain 3F (MLC3F) [126]. Most recently, the group of Pura Muñoz-Cánoves has demonstrated through isoform-specific knockout in mice that p38α is absolutely critical for the differentiation of primary myoblasts, while β and δ are not necessary for either differentiation or cardiotoxin-induced regeneration, and the γ isoform appears necessary only for optimal fusion of myoblasts [127–129]. It should be noted, however, that a discrepancy may exist between primary myoblasts and C2C12 cells, as the α, β and γ isoforms all appear to be essential for C2C12 differentiation [125, 130, 131], highlighting that the model system being used always needs to be taken into consideration.

Cell-cell contact in myoblast cultures triggers precocious differentiation, and contact is at least one mechanism by which p38α is activated (Figure 3). N-cadherin ligation between cells initiates the formation of a complex that includes the cell surface protein Cdo and scaffolding proteins that recruit p38 in addition to other components [132–134]. Precisely how this complex results in p38 activation is not known, but complex recruitment of the GTPase Cdc42 is required for p38 phosphorylation. However, as noted by Kang et al.[133], although Cdo complex formation appears to be a major mechanism behind p38 activation in differentiating myoblasts, it is likely not the only mechanism, as there are additional ways to activate p38 in the absence of Cdo complex components. Transforming growth factor β-activated kinase 1 (TAK1) is an upstream activating MAP3K that is essential for myoblast differentiation in a p38-dependent manner [135], and activation of this kinase is traditionally associated with transforming growth factor (TGF) stimulation as opposed to N-cadherin ligation. TAK1 can phosphorylate and activate MAP kinase kinase 3/6 (MKK3/6), and numerous studies have demonstrated a requirement for MKK3/6 activity in the initiation of myoblast differentiation, again in a p38-dependent manner. Whether N-cadherin ligation and Cdo are coupled to TAK1 and MKK3/6 is not known, and so it is not possible to present a clear pathway for p38 activation during differentiation.

Once activated, p38 is involved in multiple prodifferentiation processes (Figure 3). It has a powerful ability to trigger cell cycle exit, and can even force cell cycle exit in rhabdomyosarcoma cells [136]. The mechanisms by which it does so have not been well elucidated, but it can downregulate canonical proliferation markers such as cyclins A, D and E, as well as phosphorylated Rb [127, 128, 137]. Chromatin remodelling is a candidate mechanism by which p38 activity might trigger the downregulation of cell cycle-related genes. p38 can phosphorylate the histone-lysine N-methyltransferase EZH2, the catalytic subunit of the polycomb repressive complex 2 (PRC2), with phosphorylation of EZH2 necessary for PRC2's association with the transcriptional repressor YY1 and subsequent chromatin remodelling [138]. One target of this complex in myoblasts is the Pax7 promoter, and downregulation of Pax7 is a necessary step before differentiation can occur.

At the same time as p38 creates a repressive chromatin environment for Pax7 and possibly other genes, it creates a permissive environment at myogenic loci. p38 phosphorylates the BAF60 subunit of the SWI-SNF chromatin remodelling complex, and p38 recruits this complex to differentiation-specific loci [137, 139]. Through phosphorylation of MEF2D, p38 recruits an Ash2l-containing complex to myogenic loci during differentiation, which results in the marking of these genes for expression [140]. As a permissive environment is created at these loci, p38 further stimulates gene expression through the phosphorylation of additional myogenic transcription factors, including MEF2C [95, 123, 126] and E47 [141]. Phosphorylation of MEF2C is necessary for its transcriptional activation, and E47 phosphorylation allows heterodimerisation with and activation of MyoD. p38 also plays a critical role in activating other myogenic factors. Nuclear translocation of p65 during differentiation is p38-dependent [142], as is MyoD activity [95, 123, 125, 127, 136], partly through E47 phosphorylation and heterodimerisation but likely via other means as well. Ultimately, through these and possibly other mechanisms, p38 has the ability to affect the expression of a multitude of genes. Some of those directly relevant to differentiation and not already mentioned include Akt [143, 144], caveolin 3 [124] and IGF2 [145].

The responsibility of p38 during myoblast differentiation is not limited to gene regulation, but includes a critical role in other processes as well. Briata et al.[146] showed that p38 phosphorylates the mRNA decay-promoting KH type-splicing regulatory protein (KSRP). Phosphorylation prevents KSRP from associating with select transcripts, resulting in transcript stabilization, and in the case of differentiating myoblasts this allows for the accumulation of mRNA for at least two very critical myogenic proteins: the CDK inhibitor p21 and myogenin. Also, current work from our own laboratory shows that during myoblast differentiation active p38 accumulates in the cytoplasm and can phosphorylate dozens of cytosolic proteins with a variety of known functions, suggesting that the role of p38 during myogenesis likely goes far beyond gene regulation (JDR Knight, R Tian, REC Lee, F Wang, H Zou, LA Megeney, D Figeys, R Kothary, unpublished work).

Finally, it needs to be mentioned that the literature is not in complete consensus regarding the role of p38 during myoblast differentiation. A potentially conflicting result was published by Weston et al.[147], who showed that inhibiting p38α/β in a mixed culture of primary limb mesenchymal cells supports and accelerates the terminal differentiation of myocytes. Specifically, cells that already express myosin heavy chain appear to undergo accelerated fusion and/or hypertrophy, along with an increased expression of myogenic markers following p38 inhibition. These results suggest that in this type of heterogeneous environment, p38 activity, in concert with a particular milieu of factors released by nonmyogenic cells, may serve to restrict the late stages of myocyte differentiation, or that obstructing p38 activity in nonmyogenic cells present in the coculture results in the release of potent myogenic factors that drive terminal myocyte differentiation even in the absence of active p38. As no further work has been done on this model system and more experiments are required, it is not possible to reconcile these findings with the extensive data produced using other models.

Akt (IGFR, GSK3β, mTOR, S6K)

The protein kinase Akt first became known as the product of the oncogenic v-akt gene of the Akt8 murine retrovirus [148]. The retroviral oncogene has three mammalian cellular homologues (Akt1, Akt2 and Akt3) that code for a protein kinase with an N-terminal pleckstrin homology (PH) domain [148–153]. Owing to its independent discovery by three separate groups, it has two additional names: protein kinase B (PKB) and the related to the A and C kinases (RAC-PK), on the basis of its homology [149, 151].

Akt forms part of a well-studied pathway (Figure 3), and for a review, see, for example, the articles by Glass [154] and Franke [155]. This pathway mediates the effects of insulin and IGF and includes several kinases that shall be discussed together here, although focus is placed on Akt. The pathway is activated by the binding of IGF to the IGF receptor (or of insulin to the insulin receptor), and, like most growth factor receptors, IGFR contains a tyrosine kinase domain that autoactivates upon ligand binding. A principal target of IGFR is the insulin receptor substrate (IRS), which, when phosphorylated, recruits the lipid kinase phosphatidylinositol 3-kinase (PI3K) through the SH2 domain of its regulatory subunit (p85), triggering activation of the catalytic subunit. PI3K produces the membrane-bound phosphatidylinositol (3,4)-bisphosphate (PI(3,4)P₂) and phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P₃) from PI(4)P and PI(4,5)P₂, respectively. These phosphoinositide products localise PH domain-containing proteins to the plasma membrane, including Akt, the constitutively active phosphoinositide-dependent kinase 1 (PDK1), and "PDK2". The colocalisation of these kinases allows for PDK1 and PDK2 to phosphorylate Akt at distinct sites, with both phosphorylations necessary for activation. PDK2 is not a single kinase but rather a group of kinases [156], any one of which has the ability to phosphorylate Akt at the required site. Two major kinase targets of Akt are the mammalian target of rapamycin (mTOR) and glycogen synthase kinase 3β (GSK3β). Phosphorylation of mTOR by Akt activates it, resulting in an increase in protein synthesis, while Akt's phosphorylation of GSK3β inactivates this kinase, thereby removing the restraint that GSK3β places on differentiation and hypertrophy. One final, well-characterized member of this pathway is the ribosomal protein S6 kinase 1 (S6K), which is phosphorylated and activated by mTOR to positively and further regulate protein translation.

This pathway, with Akt at its heart, is activated by IGF or insulin stimulation, but there is evidence to suggest that Akt can be activated by other mechanisms in muscle cell lines. Elia et al.[157] showed that Sonic hedgehog (SHH) can stimulate Akt phosphorylation and myogenic gene expression, and, similar to work done on the p38 pathway, Bae et al.[158] showed that Akt can be activated from cell-cell contact through Cdo activation and the recruitment of the Akt-interacting partner APPL1. There is evidence to suggest that APPL1 may function downstream of insulin in myoblasts [159, 160], indicating that cell-cell contact and insulin/IGF may cooperate to activate Akt. Whether SHH also cooperates with this pathway or stimulates one in parallel is unclear, but there is certainly more to be discovered about the mechanisms of Akt activation.

While the pathway of Akt activation requires additional elaboration, the importance of the IGF-Akt axis to myogenesis cannot be debated. It has been demonstrated in culture that IGF is critical to, and a potent stimulator of, myoblast differentiation and hypertrophy, and that muscle cell lines upregulate IGF2 upon differentiation [87, 161–167]. These results carry over in vivo, as IGF overexpression in mice triggers myoblast differentiation, myofibre hypertrophy and regeneration [168–170]. Several studies have shown that Akt activity is induced during myoblast differentiation, and that its activity is critical for the induction of differentiation and hypertrophy both in culture and in vivo[52, 94, 125, 144, 171–175]. IGF can also have a positive effect on myoblast proliferation under certain conditions, and Akt may be critical for proliferation as well [176, 177], although the details regarding this pathway are poorly understood. We shall discuss the proliferative capabilities of IGF and Akt in greater detail below after first introducing the different Akt isoforms and their respective myogenic responsibilities.

IGF can activate any of the three Akt isoforms, and currently both Akt1 and Akt2 have been implicated in myogenesis, while Akt3 has not. There is very strong evidence to suggest that isoforms 1 and 2 are required at different stages, although how their activation is differentially controlled is unknown. Protein levels of Akt1 remain constant from proliferating to differentiating cells, whereas the levels and activity of Akt2 increase with differentiation [143, 176, 178–181]. Consistent with these observations, Akt2 drives differentiation [176, 177, 181–183], while Akt1 appears critical to myoblasts for proliferation but is dispensable for differentiation and may even be inhibitory to the latter process when activated alone [176, 177, 183, 184]. Conversely, Akt2 is dispensable for proliferation and cannot rescue Akt1 knockdown in proliferating myoblasts [176, 177]. It should be noted, however, that overexpression of a constitutively active mutant of either isoform can initiate and drive differentiation, but this is likely an artefact that results from artificially elevated Akt levels. It is difficult to be conclusive at the moment, especially as little work has been done in vivo or in primary cells, but there is certainly strong evidence to support distinct roles for Akt1 and Akt2 during myogenesis.

When myoblasts are initially treated with IGF, there is a proliferative response and differentiation is prevented [67, 72, 74, 177, 185–187]. This response is induced largely when myoblasts are subconfluent and is mediated in part by IGF-induced phosphorylation of ERK1/2, as well as Akt1 (Figure 2). Few targets of Akt1 in proliferative myoblasts are known, but once activated, Akt1 phosphorylates the cyclin kinase inhibitor p21, triggering its dissociation from CDK2 and leading to cell cycle progression [74, 177]. Akt can also phosphorylate forkhead box protein O1 (FoxO1) in myoblasts, with phosphorylation blocking nuclear translocation of the transcription factor and inhibiting expression of FoxO1-regulated transcripts such as the CDK inhibitor p27[187]. Evidence suggests that this IGF proliferative pathway can be turned off either by inhibiting ERK1/2, or through the activation of Akt2 [72, 94, 99, 177]. Once confluent, cell-cell contact is known to antagonize ERK1/2 activation in other cell types [188–191], and in myoblasts confluency induces p38 activity as described in the previous section, which in turn leads to the upregulation of Akt2 transcript levels [143]. Contrary to Akt1, Akt2 interacts with p21 but does not phosphorylate it, and instead appears to prevent phosphorylation by Akt1 [177]. This Akt2-p21 complex can then inhibit CDK2 and allow cell cycle exit and differentiation. Hence the switch from an IGF-induced proliferative response to an induction of differentiation may be controlled largely by the degree of cell-cell contact present.

Once Akt2 becomes activated, it triggers myoblast cell cycle exit (Figure 3). It does so by phosphorylating the pituitary homeobox 2 (Pitx2) transcription factor (which cannot be phosphorylated by Akt1) [192]. Pitx2 interacts with the mRNA binding protein HuR to stabilize cyclin D1 transcript levels to maintain proliferation, while Akt2 phosphorylation of Pitx2 causes dissociation of this complex and degradation of cyclin D1 mRNA. Once cell cycle exit has occurred, Akt's phosphorylation (and inhibition) of the FoxO family of transcription factors now allows differentiation to occur [193], as these transcription factors, although apparently necessary for cell cycle exit in myoblasts [187], are inhibitory to differentiation. Akt activity is also critical for the production of myogenic transcripts, partly through positive regulation of MyoD and MEF2C transcriptional activities [172, 181, 194]. Akt can phosphorylate the transcriptional coactivator p300, which results in the formation of an active p300-MyoD complex [137]. In myoblasts, MyoD and MEF2 activities are suppressed, partly from being bound to the transcriptional repressor prohibitin 2 (PHB2). Akt2 can remove this repression through binding to and downregulating PHB2 [183, 195], allowing MyoD and MEF2 transcriptional activation, although whether Akt2-mediated phosphorylation of PHB2 occurs is unknown. A further factor that triggers differentiation in response to Akt activity is the phosphorylation and inactivation of GSK3β. When active, GSK3β represses myoblast differentiation and fusion [182, 196–198] through inhibitory phosphorylations of β-catenin and NFATC3. The silencing of GSK3β activity by Akt allows for the accumulation and nuclear translocation of β-catenin [182, 198], resulting in the activation of the TCF/LEF family of transcription factors. GSK3β's phosphorylation of NFATC3 hides this transcription factor's nuclear localisation signal, thereby preventing nuclear accumulation and transcription of its dependent genes, while Akt's phosphorylation and inhibition of GSK3β allows this NFATC3 accumulation to occur [197, 198]. The end result of GSK3β inactivation is the activation of transcription factors that initiate the production of numerous myogenic transcripts.

Following commitment to differentiation, Akt is further required for the growth/hypertrophy of myotubes (Figure 3). Its multifaceted role during hypertrophy is emphasized by the fact that the exogenous overexpression of myogenic factors such as MyoD or myogenin cannot compensate for the absence of Akt activity during this process [199]. During hypertrophy, Akt is still responsible for phosphorylating and inactivating GSK3β as it was at the onset of differentiation, as GSK3β is inhibitory to both stages of myogenesis [173, 198, 200, 201]. Similarly, Akt's phosphorylation of the FoxO family of transcription factors is necessary not just for differentiation but also for hypertrophy. FoxO activity stimulates expression of the atrophy-inducing, muscle-specific ubiquitin ligases MAFbx and MuRF1 [202, 203], and Akt therefore blocks the expression of these ligases. One of the most well-studied downstream targets of Akt in muscle is mTOR, whose phosphorylation and activity are induced during hypertrophy [204]. mTOR itself is in fact required for both differentiation and hypertrophy [72, 122, 174], but its kinase activity is required only for the latter [204–209]. Approximately 90% of the genes regulated by IGF in differentiating myoblasts are mTOR-dependent [210], emphasizing the importance of this kinase as a hypertrophic IGF effector. mTOR, together with S6K, an mTOR substrate whose activity is induced during and critical to hypertrophy [72, 108, 122, 175, 204, 205, 209, 211], trigger protein synthesis and growth by initiating cap-dependent translation. mTOR activity also leads to the upregulation of miR-1, which inhibits HDAC4 expression, thereby allowing the upregulation of critical myogenic genes, including the profusion protein follistatin [212].

A substantial amount of research on the IGF-Akt signalling axis has been conducted, and we have briefly summarized it here. Further research regarding the distinct roles of Akt1 and Akt2 is required: if, in fact, there is a distinction; how they are differentially regulated; and the similarities and differences between downstream targets. Over 100 Akt substrates are known, but very few have been studied during myogenesis. We have described the handful of substrates that have been studied, but this must be a very incomplete picture, and so there is still much room for further exploration.